引用本文:
李霞, 熊海蓉, 蒋利华, 文祝友, 熊远福. HPLC法同时测定石蒜中加兰他敏和石蒜碱[J]. 应用化学,
2010, 27(11): 1362-1364.
doi:
10.3724/SP.J.1095.2010.00002
Citation: LI Xia, XIONG Hai-Rong, JIANG Li-Hua, WEN Zhu-Youd, XIONG Yuan-Fu. Determination of Galanthamine and Lycorine in Lycoris Herb. by HPLC[J]. Chinese Journal of Applied Chemistry, 2010, 27(11): 1362-1364. doi: 10.3724/SP.J.1095.2010.00002
Citation: LI Xia, XIONG Hai-Rong, JIANG Li-Hua, WEN Zhu-Youd, XIONG Yuan-Fu. Determination of Galanthamine and Lycorine in Lycoris Herb. by HPLC[J]. Chinese Journal of Applied Chemistry, 2010, 27(11): 1362-1364. doi: 10.3724/SP.J.1095.2010.00002
HPLC法同时测定石蒜中加兰他敏和石蒜碱
English
Determination of Galanthamine and Lycorine in Lycoris Herb. by HPLC
Abstract:
A high performance liquid chromatographic method was developed for simultaneous determining the contents of galanthamine and lycorine in Lycoris Herb. The chromatographic analysis was carried out on a phenomenex-C18 column. The mobile phase was acetonitrile (A)-0.1% trifluoroacetic acid(TFA) (B) with gradient elution. The detection wavelength was set at 289 nm. The calibration curves for galanthamine and lycorine showed good linearity in the range of 0.5~10 mg/L(r=0.9999). The detection limits were 0.09 mg/L for galanthamine and 0.15 mg/L for lycorine at the signal-to-noise ratio of 3,and the average recoveries were 99.53% and 96.82%,respectively. The method is simple,rapid,accurate and suitable for the determination of galanthamine and lycorine in Lycoris Herb.
-
Key words:
- HPLC
- / Lycoris Herb
- / galanthamine
- / lycorine
-
-
扫一扫看文章
计量
- PDF下载量: 2
- 文章访问数: 815
- HTML全文浏览量: 56
下载:
