引用本文:
姚家彪, 赵颖, 潘伟, 蒋施, 钟钰, 金雁. 蔬菜和食用菌中氨基甲酸酯类农药残留检测技术[J]. 应用化学,
2010, 27(4): 488-493.
doi:
10.3724/SP.J.1095.2010.90362
Citation: YAO Jia-Biao, ZHAO Ying, PAN Wei, JIANG Shi, ZHONG Yu, JIN Yan. Determination of Carbamate Pesticide Residues in Vegetables and Mushrooms[J]. Chinese Journal of Applied Chemistry, 2010, 27(4): 488-493. doi: 10.3724/SP.J.1095.2010.90362
Citation: YAO Jia-Biao, ZHAO Ying, PAN Wei, JIANG Shi, ZHONG Yu, JIN Yan. Determination of Carbamate Pesticide Residues in Vegetables and Mushrooms[J]. Chinese Journal of Applied Chemistry, 2010, 27(4): 488-493. doi: 10.3724/SP.J.1095.2010.90362
蔬菜和食用菌中氨基甲酸酯类农药残留检测技术
摘要:
建立了丙酮和乙腈(体积比85:15)提取,凝胶渗透色谱(GPC)法和PSA(N-丙基乙二胺)固相萃取柱2种方法净化,超高效液相色谱-串联质谱(UPLC-MS/MS)同时检测菠菜、黄瓜、大葱、蘑菇、香菇、木耳、牛肝菌和胡萝卜中19种氨基甲酸酯类农药(NMCs),同位素稀释法定量。实验结果表明,GPC法净化,回收率为70.1%~95.7%;PSA固相萃取柱净化,回收率为70.2%~94.9%。方法的检出限均为1×10-5g/kg。各种NMCs在确定的添加范围内线性关系良好,线性相关系数r均>0.99,所建立的检测方法实用、准确、灵敏度高。
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关键词:
- GPC
- / PSA
- / 超高效液相色谱-串联质谱
- / 氨基甲酸酯
English
Determination of Carbamate Pesticide Residues in Vegetables and Mushrooms
Abstract:
A method was developed for the simultaneous determination of 19 carbamate pesticides residues in spinach, cucumber, scallion, fungus, mushroom, agaric and carrot by ultra performance liquid chromatography tandem mass spectrometry(UPLCMS/MS) combined with isotope internal standard method. The vegetable and mushroom samples were extracted with V(acetone):V(acetonitrile)=85:15 then purified by gel permeation chromatography(GPC) or solid phase extraction(SPE). The recoveries ranged from 70.1% to 95.7% with the cleaning method of GPC and from 70.2% to 94.9% with the cleaning method of primary-secondary amine(PSA) SPE. The limits of detection(LOD) were 1×10-5g/kg. The method showed a good linear relationship with the correlation coefficients over 0.99. This method is practical,accurate and highly sensitive.
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Key words:
- GPC
- / PSA
- / UPLC-MS/MS
- / carbamate
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