基于g-C<sub>3</sub>N<sub>4</sub>纳米片、铜离子和邻苯二胺构建的比率荧光探针检测辅酶A

引用本文: 张悦, 张薇, 王兴华, 马品一, 孙颖, 宋大千. 基于g-C₃N₄纳米片、铜离子和邻苯二胺构建的比率荧光探针检测辅酶A[J]. 分析化学, 2022, 50(1): 64-72. doi: 10.19756/j.issn.0253-3820.210717 shu

Citation: ZHANG Yue, ZHANG Wei, WANG Xing-Hua, MA Pin-Yi, SUN Ying, SONG Da-Qian. A Ratio Fluorescent Probe Based on g-C₃N₄ Nanosheets, Copper Ion and o-Phenylenediamine for Detection of Coenzyme A[J]. Chinese Journal of Analytical Chemistry, 2022, 50(1): 64-72. doi: 10.19756/j.issn.0253-3820.210717 shu

基于g-C₃N₄纳米片、铜离子和邻苯二胺构建的比率荧光探针检测辅酶A

吉林大学化学学院, 长春 130012

通讯作者: 宋大千,E-mail:songdq@jlu.edu.cn

基金项目:
国家自然科学基金项目（No.22074052）资助。

摘要: 开发了一种基于g-C₃N₄纳米片、铜离子（Cu²⁺）和邻苯二胺（OPD）的比率荧光探针，用于检测辅酶A （Coenzyme A，CoA）。分别采用g-C₃N₄纳米片和Cu²⁺氧化OPD后的产物2，3-二氨基吩嗪（oxOPD）作为荧光指示剂，Cu²⁺和oxOPD作为淬灭剂，检测体系中g-C₃N₄纳米片的荧光被淬灭，oxOPD荧光增强。CoA与Cu²⁺形成络合物，减少了体系中作为氧化剂和淬灭剂的游离Cu²⁺的含量，导致OPD的氧化过程受到抑制，oxOPD的含量减少，荧光强度降低，g-C₃N₄纳米片的荧光恢复。以g-C₃N₄纳米片的荧光发射强度与oxOPD的荧光发射强度比（F₄₄₀/F₅₆₀）作为响应信号，用于比率荧光检测。在最佳实验条件下，采用本方法检测CoA，在1.0~100.0 μmol/L浓度范围内具有良好的比率荧光响应，线性方程为F₄₄₀/F₅₆₀=0.00502C_C_oA（μmol/L）+0.1079，R²=0.984，检出限（LOD，3 S/m）为0.6 μmol/L。此比率荧光检测体系具有良好的选择性，人血清中CoA的加标回收实验结果表明，此体系在复杂生物基质检测中具有良好的应用潜力。

关键词:

English

A Ratio Fluorescent Probe Based on g-C₃N₄ Nanosheets, Copper Ion and o-Phenylenediamine for Detection of Coenzyme A

College of Chemistry, Jilin University, Changchun 130012, China

Corresponding author: SONG Da-Qian, songdq@jlu.edu.cn

Received Date: 31 August 2021
Revised Date: 19 November 2021
Fund Project:
Supported by the National Natural Science Foundation of China (No.22074052).

Abstract: A detection system based on g-C₃N₄ nanosheets, copper ions and o-phenylenediamine (OPD) was developed for detection of coenzyme A (CoA). By using chemically inert g-C₃N₄ nanosheets and oxOPD as fluorescent indicators, CoA formed a complex with Cu²⁺, which reduced the amount of free Cu²⁺ as an oxidant and quencher in the system, resulting in the OPD oxidation process being affected. Inhibition reduced the amount of oxOPD that was used as a quencher and a fluorescent indicator, resulting in the recovery of the fluorescence of g-C₃N₄ nanosheets, thereby changing the fluorescence intensity of the entire detection system. The ratio of the fluorescence emission intensity of g-C₃N₄ nanosheets to that of oxOPD (F₄₄₀/F₅₆₀) was used as the response signal for proportional fluorescence detection. Under the optimized experimental conditions, this method had a good proportional fluorescence response toward the concentration of CoA from 1.0 μmol/L to 100.0 μmol/L. The linear regression equation was F₄₄₀/F₅₆₀=0.00502C_CoA (μmol/L)+0.1079 (R²=0.984). The detection limit (LOD=3 S/m) was 0.6 μmol/L. The constructed detection system had good selectivity, and was used to detect CoA in human serum samples, which proved that the system had the potential for detection of CoA in complex biological matrices.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于g-C₃N₄纳米片、铜离子和邻苯二胺构建的比率荧光探针检测辅酶A

通讯作者: 宋大千,E-mail:songdq@jlu.edu.cn

English

A Ratio Fluorescent Probe Based on g-C₃N₄ Nanosheets, Copper Ion and o-Phenylenediamine for Detection of Coenzyme A

Corresponding author: SONG Da-Qian, songdq@jlu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于g-C3N4纳米片、铜离子和邻苯二胺构建的比率荧光探针检测辅酶A

通讯作者: 宋大千,E-mail:songdq@jlu.edu.cn

English

A Ratio Fluorescent Probe Based on g-C3N4 Nanosheets, Copper Ion and o-Phenylenediamine for Detection of Coenzyme A

Corresponding author: SONG Da-Qian, songdq@jlu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于g-C₃N₄纳米片、铜离子和邻苯二胺构建的比率荧光探针检测辅酶A

A Ratio Fluorescent Probe Based on g-C₃N₄ Nanosheets, Copper Ion and o-Phenylenediamine for Detection of Coenzyme A

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