双胶束电动毛细管色谱测定复方化学消毒剂中聚六亚甲基单胍、聚六亚甲基双胍、醋酸洗必泰和苄索氯铵

引用本文: 刘文叶, 乔宏, 赵珊, 李疆, 丁晓静. 双胶束电动毛细管色谱测定复方化学消毒剂中聚六亚甲基单胍、聚六亚甲基双胍、醋酸洗必泰和苄索氯铵[J]. 色谱, 2016, 34(3): 332-339. doi: 10.3724/SP.J.1123.2015.10029 shu

Citation: LIU Wenye, QIAO Hong, ZHAO Shan, LI Jiang, DING Xiaojing. Determination of polyhexamethylene guanide, polyhexamethylene biguanide, chlorhexidine acetate and benzethonium chloride in compound chemical disinfectants by micellar electrokinetic chromatography with bi-micelle[J]. Chinese Journal of Chromatography, 2016, 34(3): 332-339. doi: 10.3724/SP.J.1123.2015.10029 shu

双胶束电动毛细管色谱测定复方化学消毒剂中聚六亚甲基单胍、聚六亚甲基双胍、醋酸洗必泰和苄索氯铵

刘文叶 ^1,2, ,
乔宏 ^2, ,
赵珊 ^2, ,
李疆 ^2, ,
丁晓静 ^{1,2,
,}

1.
1. 首都医科大学公共卫生学院, 北京 100069;
2.
2. 北京市疾病预防控制中心, 食物中毒诊断溯源技术北京市重点实验室, 北京 100013

通讯作者: 丁晓静

基金项目:
北京市卫生系统高层次卫生技术人才培养项目(2013-2-036). (2013-2-036)

摘要: 建立了双胶束电动毛细管色谱(MEKC)分离测定复方化学消毒剂中有效成分聚六亚甲基单胍(PHMG)、聚六亚甲基双胍(PHMB)、醋酸洗必泰(CHA)及苄索氯铵(BTC)的新方法。以50.2 cm(有效长度:40 cm)×50 μm i.d.未涂层熔融石英毛细管为分离柱,20 mmol/L硼砂+30 mmol/L十二烷基硫酸钠(SDS)+5 mmol/L脱氧胆酸钠(SD)+0.8 g/L聚乙二醇 20000为分离缓冲溶液。详细研究了分离缓冲溶液中各组分浓度、样品提取液对分离的影响。4种物质的检出限和定量限均分别为1 mg/L和3 mg/L。4种物质的校正峰面积与相应质量浓度在3~140 mg/L范围内,均具有良好的线性关系,相关系数均大于0.999。回收率在84.1%~109.6%间,相对标准偏差(RSD)均低于6%。用该法测定了11件复方化学消毒剂样品中PHMG、PHMB、CHA和BTC,与产品标识值基本吻合。该法可成功区分单胍与双胍,且操作简单,适用于消毒产品的质量监督。

关键词:

English

Determination of polyhexamethylene guanide, polyhexamethylene biguanide, chlorhexidine acetate and benzethonium chloride in compound chemical disinfectants by micellar electrokinetic chromatography with bi-micelle

1.
1. College of Public Health, Capital Medical University, Beijing 100069, China;
2.
2. Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Diseases Control and Prevention, Beijing 100013, China

Corresponding author: 丁晓静

Received Date: 19 October 2015
Fund Project:
北京市卫生系统高层次卫生技术人才培养项目(2013-2-036).

Abstract: A new method for the accurate assay of four components (polyhexamethylene guanide (PHMG), polyhexamethylene biguanide (PHMB), chlorhexidine acetate (CHA) and benzethonium chloride (BTC)) in compound chemical disinfectants by micellar electrokinetic chromatography (MEKC) with bi-micelle was developed. An uncoated fused-silica capillary with 50 μm i.d. and 50.2 cm total length (effective length: 40 cm) under a separation voltage of 24 kV was used. The separation buffer consisted of 20 mmol/L Na₂B₄O₇, 30 mmol/L sodium dodecyl sulfate (SDS), 5 mmol/L sodium deoxycholate (SD) and 0.8 g/L polyethyleneglycol (PEG) 20000. A 1 : 10 dilution of the running buffer with ultrapure water was used as the sample buffer. The injection pressure and time were 3.448 kPa and 12 s, respectively. Liquid samples could be directly injected after dilution with sample buffer. Solid samples were extracted with 10 mL of sample buffer twice and the two extracted solutions were merged together and then directly injected. The factors such as the buffer concentration and pH, the concentrations of SDS and SD, the concentration of PEG 20000 and the sample extraction solution which influence the separation were investigated in detail. The corrected peak areas versus the concentrations of the four components showed good linear relationships within the range from 3 mg/L to 140 mg/L with correlation coefficients (r) greater than 0.999. The limits of detection (LODs) and the limits of quantification (LOQs) of the four components were all 1 mg/L and 3 mg/L, respectively. The average recoveries at three spiked levels were in the range of 84.1%-109.6% with RSDs all less than 6%. Eleven samples were analyzed and the results met well with the specified content. The PHMG could be easily discriminated from PHMB. The MEKC method is suitable for the supervision of compound chemical disinfectants.

Key words:

micellar electrokinetic chromatography (MEKC)
/ polyhexamethylene guanide (PHMG)
/ polyhexamethylene biguanide (PHMB)
/ chlorhexidine acetate (CHA)
/ benzethonium chloride (BTC)
/ compound chemical disinfectant

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沈阳化工大学材料科学与工程学院沈阳 110142

双胶束电动毛细管色谱测定复方化学消毒剂中聚六亚甲基单胍、聚六亚甲基双胍、醋酸洗必泰和苄索氯铵

通讯作者: 丁晓静

English

Determination of polyhexamethylene guanide, polyhexamethylene biguanide, chlorhexidine acetate and benzethonium chloride in compound chemical disinfectants by micellar electrokinetic chromatography with bi-micelle

Corresponding author: 丁晓静

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

双胶束电动毛细管色谱测定复方化学消毒剂中聚六亚甲基单胍、聚六亚甲基双胍、醋酸洗必泰和苄索氯铵

通讯作者: 丁晓静

English

Determination of polyhexamethylene guanide, polyhexamethylene biguanide, chlorhexidine acetate and benzethonium chloride in compound chemical disinfectants by micellar electrokinetic chromatography with bi-micelle

Corresponding author: 丁晓静

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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