多重数字聚合酶链式反应技术及其应用

引用本文: 尹居鑫, 夏丽萍, 邹哲宇, 牟颖. 多重数字聚合酶链式反应技术及其应用[J]. 分析化学, 2022, 50(1): 25-38. doi: 10.19756/j.issn.0253-3820.210530 shu

Citation: YIN Ju-Xin, XIA Li-Ping, ZOU Zhe-Yu, MU Ying. Multiplex Digital Polymerase Chain Reaction Technology and Its Application[J]. Chinese Journal of Analytical Chemistry, 2022, 50(1): 25-38. doi: 10.19756/j.issn.0253-3820.210530 shu

多重数字聚合酶链式反应技术及其应用

尹居鑫 ^1,2, ,
夏丽萍 ^2, ,
邹哲宇 ^2, ,
牟颖 ^{2,
,}

1.
浙大城市学院, 信息与电气工程学院, 杭州 310015;
2.
浙江大学工业控制技术国家重点实验室, 分析仪器研究中心, 杭州 310027

通讯作者: 牟颖,E-mail:muying@zju.edu.cn

基金项目:
国家重点研发计划项目（No.2019YFE0103900）和国家自然科学基金项目（No.32071481）资助。

摘要: 数字聚合酶链式反应（PCR）作为一种具有广泛应用前景的核酸检测技术，已经成为当前公认的最为精准的核酸检测技术之一。多重检测是数字PCR发展的重要方向，随着微流控技术的发展，多重数字PCR技术已越来越成熟。本文综述了近年来多重数字PCR的研究进展，重点归纳总结了近五年来多重数字PCR技术的实现方式，介绍了多重数字PCR技术在液体活检、转基因检测、新冠病毒检测等热点领域的应用。最后，对多重数字PCR技术面临的问题和挑战进行了探讨，并展望了该技术未来的发展方向。

关键词:

English

Multiplex Digital Polymerase Chain Reaction Technology and Its Application

1.
School of Information and Electrical Engineering, Zhejiang University City College, Hangzhou 310015, China;
2.
Research Center for Analytical Instrumentation, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310027, China

Corresponding author: MU Ying, muying@zju.edu.cn

Received Date: 28 May 2021
Revised Date: 29 July 2021
Fund Project:
Supported by the National Program on Key Research Project of China(No.2019YFE0103900) and the National Natural Science Foundation of China (No.32071481).

Abstract: Digital polymerase chain reaction (PCR), as a nucleic acid detection technology with wide application prospect, has become one of the most accurate nucleic acid detection technology at present. Multiplex detection is an important direction for the development of digital PCR technique. With the development of microfluidic technology, multiplex digital PCR technique has become more and more mature. This paper reviewed the research progresses of multiplex digital PCR in recent years, especially summarized the implementation of multiplex digital PCR technique in the past five years, and introduced the application of multiplex digital PCR technique in hot areas such as liquid biopsy, transgenic detection, and SARS-Cov-2 detection. Finally, the issues and challenges faced by multiplex digital PCR technique were discussed and the future direction of the technology was foreseen.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

多重数字聚合酶链式反应技术及其应用

通讯作者: 牟颖,E-mail:muying@zju.edu.cn

English

Multiplex Digital Polymerase Chain Reaction Technology and Its Application

Corresponding author: MU Ying, muying@zju.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

多重数字聚合酶链式反应技术及其应用

通讯作者: 牟颖,E-mail:muying@zju.edu.cn

English

Multiplex Digital Polymerase Chain Reaction Technology and Its Application

Corresponding author: MU Ying, muying@zju.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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