同步辐射微区X射线荧光光谱法原位分析蚯蚓中K,Ca,Cu,Zn和Pb

引用本文: 袁静, 罗立强. 同步辐射微区X射线荧光光谱法原位分析蚯蚓中K,Ca,Cu,Zn和Pb[J]. 分析化学, 2016, 44(5): 792-798. doi: 10.11895/j.issn.0253-3820.150801 shu

Citation: YUAN Jing, LUO Li-Qiang. Distribution Analysis of K, Ca, Cu, Zn and Pb in Earthworm by In Situ Synchrotron Radiation Micro X-Ray Fluorescence[J]. Chinese Journal of Analytical Chemistry, 2016, 44(5): 792-798. doi: 10.11895/j.issn.0253-3820.150801 shu

同步辐射微区X射线荧光光谱法原位分析蚯蚓中K,Ca,Cu,Zn和Pb

袁静 ^1,2,3, ,
罗立强 ^2,3,

1.
中国地质调查局南京地质调查中心, 南京 210016;
2.
国家地质实验测试中心, 北京 100037;
3.
中国地质大学(北京), 北京 100083
基金项目:
本文系国家自然科学基金项目(Nos.20775018,41201527),国家高新技术研究发展计划863项目(No.2007AA06Z124)、中国地质调查局地质大调查项目(No.12120113015200)、国家科技专项"汶川地震断裂带科学钻探工程"0008课题和0003课题第四专题的联合资助

摘要: 为了研究重金属元素在蚯蚓体内的富集和存储机制,使用同步辐射微区X射线荧光光谱法(μ-SRXRF)研究了K,Ca,Cu,Zn和Pb元素在生长于南京栖霞山铅锌矿区附近菜园土壤的蚯蚓体内的分布特征,发现Pb主要富集在蚯蚓后部消化道周围的区域,Zn,Cu在蚯蚓后部的分布规律与Pb类似,推测后部消化道周围区域的分布是蚯蚓为了阻断毒性重金属元素威胁而特有的富集和存储方式。5种元素的相关性表明,Pb和Zn相关性最高,存储方式最为相似,Pb与K和Ca在蚯蚓后部的分布呈显著正相关,说明毒性元素Pb在蚯蚓体内的富集和存储过程可能伴随着其它元素的吸收。本研究表明,μ-SRXRF在原位微区分析蚯蚓样品的元素空间分布方面具有很大优势,而进一步开展土壤及蚯蚓中Pb的形态研究,是研究重金属胁迫下蚯蚓解毒机制的重要前提。

关键词:

English

Distribution Analysis of K, Ca, Cu, Zn and Pb in Earthworm by In Situ Synchrotron Radiation Micro X-Ray Fluorescence

YUAN Jing ^1,2,3, ,
LUO Li-Qiang ^2,3,

1.
Nanjing Center of Geological Survey, China Geological Survey, Nanjing 210016, China;
2.
National Research Center for Geoanalysis, Beijing 100037, China;
3.
China University of Geosciences, Beijing 100083, China
Received Date: 12 October 2015
Revised Date: 25 January 2016
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 20775018, 41201527) and the National High Technology Research and Development Program of China (No. 2007AA06Z124)

Abstract: To study the accumulation and storage mechanism of heavy metals in earthworms, synchrotron radiation micro X-ray fluorescence (μ-SRXRF) was used to determine element distribution characteristics of K, Ca, Cu, Zn and Pb in earthworms living in garden around Qixiashan lead-zinc mine of Nanjing. The imaging of these elements showed that Pb primarily accumulated in the region around the posterior alimentary canal. There were also Zn and Cu signals in the anterior and posterior part of earthworm, besides the distribution characters of Zn and Cu in the posterior part of earthworm were the same as that of Pb. The findings indicated that the distribution characters in the region around the posterior alimentary canal was a special accumulation and storage mechanism in earthworms for cutting the threat of toxic heavy metals. The high relationship between Pb and Zn distribution indicated that the two elements had a very similar pathway. In this study, we proved that μ-SRXRF had a great advantage in spatial distribution analysis of elements in earthworm samples. A further research focusing on Pb speciation in soil and earthworms is needed for understanding the detoxification mechanism in earthworms under heavy metal stress.

Key words:

Synchrotron radiation micro X-ray fluorescence
/ Earthworm
/ In situ analysis
/ Lead
/ Storage mechanism

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同步辐射微区X射线荧光光谱法原位分析蚯蚓中K,Ca,Cu,Zn和Pb

English

Distribution Analysis of K, Ca, Cu, Zn and Pb in Earthworm by In Situ Synchrotron Radiation Micro X-Ray Fluorescence

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同步辐射微区X射线荧光光谱法原位分析蚯蚓中K,Ca,Cu,Zn和Pb

English

Distribution Analysis of K, Ca, Cu, Zn and Pb in Earthworm by In Situ Synchrotron Radiation Micro X-Ray Fluorescence

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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