引用本文:
刘凡, 张元勤, 张智锦. 1,4,7,10-四氮杂环十二烷合钴与牛血清白蛋白的相互作用[J]. 应用化学,
2013, 30(2): 232-238.
doi:
DOI:10.3724/SP.J.1095.2013.20140
Citation: LIU Fan, ZHANG Yuanqin, ZHANG Zhijin. Interaction of 1,4,7,10-Tetraazacyclododecane-Co(Ⅱ) with Bovine Serum Albumin[J]. Chinese Journal of Applied Chemistry, 2013, 30(2): 232-238. doi: DOI:10.3724/SP.J.1095.2013.20140
Citation: LIU Fan, ZHANG Yuanqin, ZHANG Zhijin. Interaction of 1,4,7,10-Tetraazacyclododecane-Co(Ⅱ) with Bovine Serum Albumin[J]. Chinese Journal of Applied Chemistry, 2013, 30(2): 232-238. doi: DOI:10.3724/SP.J.1095.2013.20140
1,4,7,10-四氮杂环十二烷合钴与牛血清白蛋白的相互作用
摘要:
利用荧光光谱法,研究了1,4,7,10-四氮杂环十二烷合钴(Cyclen-Co(Ⅱ))配合物与牛血清白蛋白(BSA)的相互作用,用Stern-Volmer和Lineweaver-Burk方程确定了其荧光猝灭机理为静态猝灭,计算得到了在不同温度和pH值条件下以及在聚氧乙烯(23)月桂醇醚(Brij35)、十六烷基三甲基溴化铵(CTAB)、十二烷基硫酸钠(SDS)3种表面活性剂胶束溶液中,Cyclen-Co(Ⅱ)和BSA作用的结合点位数、结合常数和热力学参数,研究结果表明,其作用力主要为氢键和范德华力。
English
Interaction of 1,4,7,10-Tetraazacyclododecane-Co(Ⅱ) with Bovine Serum Albumin
Abstract:
The interaction of Cyclen-Co(Ⅱ) with bovine serum albumin(BSA) was investigated by fluorescence method.The results show that the Cyclen-Co(Ⅱ) strongly quenches the fluorescence of BSA.It has been proved that the fluorescence quenching mechanism belongs to the static quenching by Stern-Volmer equation and Lineweaver-Burk equation.According to Lineweaver-Burk equation and thermodynamics equation,the binding constants,binding sites and thermodynamics parameters were obtained at different temperatures in the aqueous solution and in the solutions of Brij35,CTAB,SDS surfactants.The Van der Waals' forces play a main role in the binding of Cyclen-Co(Ⅱ) with BSA.
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