引用本文:
安玲玲, 常颖萃, 杜江燕. 三维有序石墨烯掺杂纳米二氧化钛酶生物传感器[J]. 应用化学,
2013, 30(2): 171-177.
doi:
DOI:10.3724/SP.J.1095.2013.20148
Citation: AN Lingling, CHANG Yingcui, DU Jiangyan. A Novel Hydrogen Peroxide Biosensor Based on the Immobilization of Catalase on Three-Dimensionally Ordered Macroporous Graphene-doped Titanium Dioxide Film[J]. Chinese Journal of Applied Chemistry, 2013, 30(2): 171-177. doi: DOI:10.3724/SP.J.1095.2013.20148
Citation: AN Lingling, CHANG Yingcui, DU Jiangyan. A Novel Hydrogen Peroxide Biosensor Based on the Immobilization of Catalase on Three-Dimensionally Ordered Macroporous Graphene-doped Titanium Dioxide Film[J]. Chinese Journal of Applied Chemistry, 2013, 30(2): 171-177. doi: DOI:10.3724/SP.J.1095.2013.20148
三维有序石墨烯掺杂纳米二氧化钛酶生物传感器
English
A Novel Hydrogen Peroxide Biosensor Based on the Immobilization of Catalase on Three-Dimensionally Ordered Macroporous Graphene-doped Titanium Dioxide Film
Abstract:
A novel hydrogen peroxide biosensor based on three-dimensionally ordered macroporous graphene-doped titanium dioxide modified ITO electrode(3DOM GR-Au-TiO2/ITO) was prepared by a sol-gel procedure using polystyrene colloidal crystals as template.Catalase has been successfully immobilized on the modified electrode.The cyclic voltammetry(CV),electrochemical impedance spectra(EIS) and chronoamperometric characterizations reveal that the modified electrode exhibits an excellent electrocatalytic activity,a wide linear range for H2O2 concentration from 3.0×10-6 mol/L to 3.6×10-3 mol/L with a detection limit of 4.2×10-7 mol/L(S/N=3),fast response,high sensitivity,good reproducibility and stability.
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Key words:
- catalase
- / graphene
- / nanometer titanium dioxide
- / biosensor
- / electrocatalysis
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