引用本文:
郑阿萍, 陈丙年, 陈发河, 王力. 钒取代硅钨氧酸盐对酪氨酸酶的抑制作用[J]. 应用化学,
2013, 30(2): 165-170.
doi:
DOI:10.3724/SP.J.1095.2013.20160
Citation: ZHENG Aping, CHEN Bingnian, CHEN Fahe, WANG Li. Inhibition Effect of Vanadate-replaced Polyoxometalates on Mushroom Tyrosinase[J]. Chinese Journal of Applied Chemistry, 2013, 30(2): 165-170. doi: DOI:10.3724/SP.J.1095.2013.20160
Citation: ZHENG Aping, CHEN Bingnian, CHEN Fahe, WANG Li. Inhibition Effect of Vanadate-replaced Polyoxometalates on Mushroom Tyrosinase[J]. Chinese Journal of Applied Chemistry, 2013, 30(2): 165-170. doi: DOI:10.3724/SP.J.1095.2013.20160
钒取代硅钨氧酸盐对酪氨酸酶的抑制作用
摘要:
测定了自制的α-1,2,3-K6H[SiW9V3O40]、α-1,2-K6[SiW10V2O40]和α-K5[SiW11VO40](以下分别简写为α-SiW9V3、α-SiW10V2和α-SiW11V)钒取代多金属氧酸盐对蘑菇酪氨酸酶活性的抑制作用。结果表明,在pH=6.8的NaH2PO4-Na2HPO4缓冲溶液中,α-SiW9V3对酪氨酸酶有较强的抑制作用,表现为对酶稳态活力的抑制作用和对迟滞时间的延长作用,在DMSO溶液中,其IC50为0.6841 mmol/L,0.7 mmol/Lα-SiW9V3可使单酚酶的迟滞时间由235 s延长至650 s,增加了2.77倍。α-SiW9V3对酪氨酸酶单酚酶的抑制为可逆作用过程,抑制类型为混合型,其抑制常数KI、KIS分别为4.22和2.39 mmol/L。α-SiW10V2不溶于DMSO,故未研究其对酶的抑制作用,α-SiW11V对酪氨酸酶抑制作用很弱。
English
Inhibition Effect of Vanadate-replaced Polyoxometalates on Mushroom Tyrosinase
Abstract:
Three kinds vanadate-replaced polyoxometalates,α-1,2,3-K6H[SiW9V3O40],α-1,2-K6[SiW10V2O40] and α-K5[SiW11VO40](abbreviated as α-SiW9V3,α-SiW10V2,α-SiW11V) were synthesized and evaluated as tyrosinase inhibitors using the enzymological kinetic method.The results demonstrate that α-SiW9V3 can inhibit tyrosinase strongly,as demonstrated by the inhibition of the steady-state activity of the enzyme and the lengthening of the lag time.The IC50 of α-SiW9V3 was estimated to be 0.6841 mmol/L for the inhibition of mushroom tyrosinase.It resulted in the lag time of the monophenolase extension from 235 s to 650 s with 0.7 mmol/L of α-SiW9V3.The inhibition of α-SiW9V3 on monophenolase of mushroom tyrosinase proceeds as reversible process,and the inhibition of α-SiW9V3 is a competitive-uncompetitive mixed-Ⅱ type due to the oxidation of L-tyrosine.The inhibition constants(KI and KIS) are determined to be 4.22 mmol/L and 2.39 mmol/L,respectively.As for the α-SiW10V2,however,it is unsoluble in DMSO buffer,and its effect on the mushroom tyrosinase cannot be studied.While the inhibition ability of α-SiW11V on tyrosinase activity is rather weak.
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Key words:
- polyoxometalates
- / mushroom tyrosinase
- / monophenolase activity
- / inhibition effect
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