引用本文:
郭晓明, 吴霞琴, 江丽萍, 章宗穰. 溶胶-凝胶固定葡萄糖氧化酶方法的改进[J]. 应用化学,
2002, 19(8): 789-791.
Citation: GUO Xiao-Ming, WU Xia-Qin, JIANG Li-Ping, ZHANG Zong-Rang. An Improved Sol-Gel Method for Glucose Oxidase Immobilization[J]. Chinese Journal of Applied Chemistry, 2002, 19(8): 789-791.
Citation: GUO Xiao-Ming, WU Xia-Qin, JIANG Li-Ping, ZHANG Zong-Rang. An Improved Sol-Gel Method for Glucose Oxidase Immobilization[J]. Chinese Journal of Applied Chemistry, 2002, 19(8): 789-791.
English
An Improved Sol-Gel Method for Glucose Oxidase Immobilization
Abstract:
An improved sol-gel method was proposed for glucose oxidase immobilization. Gelatin film was introduced onto the surface of the substrate electrode before the formation of the sol-gel membrane. The condition parameters affecting the sol-gel membrane preparation have been investi-gated and optimized. The covered gel membrane with entrapped enzyme was aged in a vessel at 4℃ for 48 h in order to prevent the membrane cracking and peeling off from the surface of substrate electrode. The fabricated glucose sensors exhibited sensitivity of 50 nA/(mmol·L-1) with linear range in range of 0.05~12 mmol/L, response time 1.5 min and satisfactory lifetime.
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Key words:
- glucose oxidase
- / sol-gel method
- / gelatin
- / biosensor
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