引用本文:
梁汝萍, 邱建丁, 邹小勇, 蔡沛祥. 基于Nafion-L-赖氨酸-溶胶-凝胶膜的电催化型多巴胺传感器[J]. 应用化学,
2003, 20(4): 346-350.
Citation: LIANG Ru-Ping, QIU Jian-Ding, ZOU Xiao-Yong, CAI Pei-Xiang. Sol-gel-coated Electrocatalytic Dopamine Sensor Based on L-Lysine Incorporated Nafion Film[J]. Chinese Journal of Applied Chemistry, 2003, 20(4): 346-350.
Citation: LIANG Ru-Ping, QIU Jian-Ding, ZOU Xiao-Yong, CAI Pei-Xiang. Sol-gel-coated Electrocatalytic Dopamine Sensor Based on L-Lysine Incorporated Nafion Film[J]. Chinese Journal of Applied Chemistry, 2003, 20(4): 346-350.
基于Nafion-L-赖氨酸-溶胶-凝胶膜的电催化型多巴胺传感器
摘要:
利用滴涂于铂盘表面的Nafion膜中负电性的磺酸基与L-赖氨酸阳离子之间的静电作用实现L-赖氨酸的固定化,再在Nafion-L-赖氨酸修饰电极表面涂布一层溶胶-凝胶薄膜,制备成Nafion-L-赖氨酸-溶胶-凝胶电催化型多巴胺传感器。采用循环伏安法和电流-时间曲线考察了传感器的电化学特性,采用差示脉冲伏安法对多巴胺进行定量分析,线性范围为5.0×10-7~5.0×10-4 mol/L,检出限为5.0×10-8 mol/L。该传感器制备方法简单、稳定性好、灵敏度高,且具有良好的选择性,能有效排除抗坏血酸等常见干扰物质对测定的影响,用于生物样品分析,结果令人满意。传感器用毕于4℃干态保存4周,其响应信号基本不变。
English
Sol-gel-coated Electrocatalytic Dopamine Sensor Based on L-Lysine Incorporated Nafion Film
Abstract:
The dopamine (DA) electrocatalytical sensor is prepared from a Nafion film coated platinum electrode by immersing it in L-lysine solution,and coated with SiO2 sol-gel layer.The L-lysine is immobilized via electrostatic force between the cation of Lys and the negatively charged sulfonic acid groups in Nafion film.Cyclic voltammetry (CV) is used to investigate the electrochemical characteristics of the sensor.The linearity of DA concentration in the range of 5.0×10-7~5.0×10-4 mol/L with detection limit of 5.0×10-8 mol/L and the correlation coefficient of 0.999 are obtained by differential pulse voltammetry (DPV).No interference was found from ascorbic acid,nitrite,uric acid,glucose and L-arginine in the determination of DA.The sensor is stable for about 4 weeks in a dry state at 4℃.Satisfactory determination results of DA in samples are obtained by this method.
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Key words:
- SiO2 sol-gel
- / L-lysine
- / nafion
- / electrocatalytical dopamine sensor
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