引用本文:
黄宝美, 姚程炜, 陈红. 鱼类中孔雀石绿残留量的高效毛细管电泳检测[J]. 应用化学,
2007, 24(3): 327-330.
Citation: HUANG Bao-Mei, YAO Cheng-Wei, CHENG Hong. Determination of Malachite Green in Fishes by Capillary Electrophoresis Combined with Conductive Detection[J]. Chinese Journal of Applied Chemistry, 2007, 24(3): 327-330.
Citation: HUANG Bao-Mei, YAO Cheng-Wei, CHENG Hong. Determination of Malachite Green in Fishes by Capillary Electrophoresis Combined with Conductive Detection[J]. Chinese Journal of Applied Chemistry, 2007, 24(3): 327-330.
鱼类中孔雀石绿残留量的高效毛细管电泳检测
摘要:
建立了鱼类中孔雀石绿残留量的高效毛细管电泳-电导法。探讨了缓冲溶液浓度、pH值及其分离电压,进样时间,有机添加剂对检测的影响。以无涂层融硅石英毛细管(75μm×50cm)为分离柱,乙酸-乙酸铵(乙酸铵浓度为12mmol/L)为电泳介质,异丙醇为有机添加剂,分离高压为20kV,pH=4.0条件下,在7.2~180mg/L范围内峰高(y)与质量浓度(c)之间呈良好的线性关系,线性系数R=0.9962,线性回归方程为:y=2.02+102c,检出限为1.8mg/L,样品加标加收率在90%~102%之间。结果表明,该法简便、快速、准确,回收率较高。
English
Determination of Malachite Green in Fishes by Capillary Electrophoresis Combined with Conductive Detection
Abstract:
A method for determination of Malachite green in fishes was established by high performance capillary electrophoresis using self-constructed conductivity detection system. Several parameters affecting the determination were studied, including concentration of buffer, value of pH, organic concentration, separation voltage, and sampling time. Under the following condions:uncoated fused silica capillary(45 cm×75 μm) as separation channel, HAC-NH4AC(concentration of NH4AC 12 mmol/L, pH=4.0) as running buffer, 20 kV as separation voltage, a linearity exists between peak height(y) and mass concentration of malachite green(c) in the range of 7.2~180 mg/L with regression equation y=2.02+102c, linearity coefficient is 0.996 2, determination limit is 1.8 mg/L and average recovery rate is from 90.0%~102.0%. The results show that the method is simple, quick, accurate and has high recovery.
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Key words:
- capillary electrophoresis
- / Malachite green
- / conductive detection
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