Abstract: A new compound material CS-MCM-48 was prepared by coating chitosan(CS) on mesoporous molecular sieve MCM-48. When the mass ratio of CS to MCM-48 was adjusted to 1:10, the specific surface area, average pore diameter and pore volume of CS-MCM-48(1:10) reached 408 m²/g, 2.14 nm and 0.38 cm³/g, respectively. The lipase(Pseudomonas Cepacia Lipase, PSL) was immobilized on the CS-MCM-48 in a mixed solvent of phosphate buffer solution and isooctane and used for one-pot reduction and transesterification synthesis of pure enantiomers from prochiral acetophenone. The results indicate that PSL immobilized on CS-MCM-48 possessed much higher catalytic transesterification activity and enantioselectivity than on single MCM-48 or CS. The enantiomeric excess of products (S)-1-phenylethanol and (R)-1-phenylethyl acetate reached respectively 18.0% and 99% with a 1-phenylethanol conversion of 15.3% and an enantioselectivity E value of 237 in toluene at 40℃, which showed the immobilized lipase PSL/CS-MCM-48 exhibited conside-rable chiral resolution properties.