引用本文:
龚美娟, 李静, 韩涛, 蔡称心, 陆天虹, 葛存旺, 杜江燕. 电化学方法检测DNA碳纳米管修饰电极[J]. 应用化学,
2008, 25(9): 1037-1041.
Citation: GONG Mei-Juan, LI Jing, HAN Tao, CAI Chen-Xin, LU Tian-Hong, GE Cun-Wang, DU Jiang-Yan. Evaluation of DNA-CNTs Modified Electrods by Electrochemical Methods[J]. Chinese Journal of Applied Chemistry, 2008, 25(9): 1037-1041.
Citation: GONG Mei-Juan, LI Jing, HAN Tao, CAI Chen-Xin, LU Tian-Hong, GE Cun-Wang, DU Jiang-Yan. Evaluation of DNA-CNTs Modified Electrods by Electrochemical Methods[J]. Chinese Journal of Applied Chemistry, 2008, 25(9): 1037-1041.
电化学方法检测DNA碳纳米管修饰电极
English
Evaluation of DNA-CNTs Modified Electrods by Electrochemical Methods
Abstract:
DNA modified carbon nanotubes electrodes are widely used in various fields and the immobilization step is of vital importance to them.Based on the carbon nanotubes(CNTs)-modified glassy carbon(GC)(CNTs/GC) electrode,two kinds of DNA-modified CNTs/GC(DNA-CNTs/GC) electrodes were fabricated by means of the absorption method and covalent bonding method.The electrochemical characteristics of the DNA-CNTs/GC electrodes were investigated via the cyclic voltammetry,differential pulse voltammetry and electrochemical impedance technique with Th as electrochemically active indicator.The results indicate that the DNA-CNTs/GC electrode prepared with the covalent bonding method possesses advantages of a large immobilization amount of DNA on the electrode surface and good stability,which could be used in the fields of electrochemical gene biosensors.
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Key words:
- DNA
- / carbon nanotube
- / modified electrode
- / thionine
- / electro-active indicator
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