一个基于NBD-NH2荧光团的增强型pH荧光探针及其细胞造影研究

李静 朱成成 何卫江

引用本文: 李静, 朱成成, 何卫江. 一个基于NBD-NH2荧光团的增强型pH荧光探针及其细胞造影研究[J]. 无机化学学报, 2013, 29(12): 2528-2534. doi: 10.3969/j.issn.1001-4861.2013.00.396 shu
Citation:  LI Jing, ZHU Cheng-Cheng, HE Wei-Jiang. A Turn-on pH Fluorescent Probe Based on NBD-NH2 Fluorophore and Its pHi Imaging Application[J]. Chinese Journal of Inorganic Chemistry, 2013, 29(12): 2528-2534. doi: 10.3969/j.issn.1001-4861.2013.00.396 shu

一个基于NBD-NH2荧光团的增强型pH荧光探针及其细胞造影研究

  • 基金项目:

    国家自然科学基金(No.21271100) (No.21271100)

    国家重点基础研究发展计划(No.2011CB935800)资助项目。 (No.2011CB935800)

摘要: 本文以NBD-NH2荧光团偶联N-(4-吡啶甲基)乙二胺质子受体构建了基于光致电子转移(PET)机制的pH探针NBD-Py。探针在pH 5.0~8.5范围内显示pH降低导致的荧光增强响应,pKa为6.48。该响应不受胞内常见金属离子干扰且有良好的可逆性。研究表明质子受体中引入4-甲基吡啶修饰乙二胺是探针实现对弱酸/近中性pH响应的关键。HeLa细胞中的造影证实了探针的pHi造影能力和溶酶体靶向性能,为溶酶体pH相关的生理过程研究提供了新的可靠手段。

English

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  • 收稿日期:  2013-05-20
  • 网络出版日期:  2013-07-16
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