聚甲基丙烯酸甲酯-牛血清白蛋白核壳纳米粒子在金表面的吸附过程及在传感器中的应用

引用本文: 何传新, 袁安朋, 张黔玲, 任祥忠, 李翠华, 刘剑洪. 聚甲基丙烯酸甲酯-牛血清白蛋白核壳纳米粒子在金表面的吸附过程及在传感器中的应用[J]. 物理化学学报, doi: 10.3866/PKU.WHXB201207191 shu

Citation: HE Chuan-Xin, YUAN An-Peng, ZHANG Qian-Lin, REN Xiang-Zhong, LI Cui-Hua, LIU Jian-Hong. Adsorption of Core-Shell Poly(Methyl Methacrylate)-Bovine Serum Albumin Nanoparticles on ld Surface and Its Sensor Application[J]. Acta Physico-Chimica Sinica, doi: 10.3866/PKU.WHXB201207191 shu

聚甲基丙烯酸甲酯-牛血清白蛋白核壳纳米粒子在金表面的吸附过程及在传感器中的应用

基金项目:
国家自然科学基金青年基金(21004040) (21004040)

深圳大学校基金(201102)资助项目 (201102)

摘要:

利用铜离子引发体系, 制备出核层为聚甲基丙烯酸甲酯(PMMA)壳层为牛血清白蛋白(BSA)的PMMA-BSA核壳纳米粒子. 通过透射电子显微镜(TEM)表征, 直接观察到PMMA-BSA纳米粒子的核壳结构.结合X射线光电子能谱(XPS)测试, 分析PMMA-BSA纳米粒子的表面成分, 证明PMMA-BSA纳米粒子的壳层是BSA. 利用带耗散的石英晶体微天平(QCM-D)研究了PMMA-BSA纳米粒子在金片表面的吸附行为. 频率的迅速下降, 耗散因子的快速上升, 说明PMMA-BSA粒子快速地吸附到金片表面. 利用磷酸盐缓冲液反复冲洗时, 频率和耗散没有变化, 表明PMMA-BSA 纳米粒子在金片上吸附较牢固. 以金电极为基底电极, 吸附PMMA-BSA纳米粒子后, 利用戊二醛修饰粒子壳层, 再通过氨基与醛基的反应来固定葡萄糖氧化酶, 制备出电流型葡萄糖传感器. 电化学测试表明该传感器对葡萄糖具有良好的电流响应, 在0.3 V的工作电位下, 响应电流与葡萄糖浓度在0.20-5.85 mmol·L^-1范围内呈现出较好的线性关系, 相关系数为0.989. 传感器的灵敏度高达28.6 μA·L·mmol^-1·cm^-2, 响应时间仅为11 s. 传感器还具有良好的稳定性, 在25℃下储存30 d, 响应电流仅下降了16%.

关键词:

English

Adsorption of Core-Shell Poly(Methyl Methacrylate)-Bovine Serum Albumin Nanoparticles on ld Surface and Its Sensor Application

1.
Shenzhen Key Laboratory of Functional Polymer, College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen518060, Guangdong Province, P. R. China
Received Date: 14 May 2012
Available Online: 17 October 2012
Fund Project:
国家自然科学基金青年基金(21004040)

深圳大学校基金(201102)资助项目

Abstract:

Core-shell poly(methyl methacrylate)-bovine serum albumin (PMMA-BSA) nanoparticles with PMMA cores and BSA shells were prepared via a copper ion-mediated initiation system. The core-shell structure of the nanoparticles was characterized by transmission electron microscopy (TEM) and the surface compositions of the nanoparticles were tested by X-ray photoelectron spectroscopy (XPS), which further demonstrated that the particles have a BSA protein shell. The adsorption of these PMMA-BSA particles onto ld surfaces was studied by quartz crystal microbalance with dissipation (QCM-D). The significantly change of frequency shift and dissipation factor indicated that PMMA-BSA particles are adsorbed on the ld surface. The repeated buffer washing had nearly no effect on either frequency shift or dissipation factor, revealing that the adsorption is fairly strong. An amperometric glucose biosensor was constructed by immobilizing glucose oxidase on PMMA-BSA particles modified with glutaraldehyde, using a ld electrode as a substrate on which to adsorb the PMMA-BSA particles. Electrochemical measurements show this biosensor exhibited a od current response to glucose. Working at 0.3 V, the biosensor had a short response time of 11 s, a sensitivity of 28.6 μA·L^-1·mmol^-1·cm^-2 and a linear range from 0.2 to 5.85 mmol·L^-1 with a correlation coefficient of 0.989. After storage at 25℃ for one month, the sensor response current decreased by only 16%, thus showing od thermal stability.

Key words:

1. [1]
  
  (1) Wang, J. Chem. Rev. 2008, 108, 814. doi: 10.1021/cr068123a
  (1) Wang, J. Chem. Rev. 2008, 108, 814. doi: 10.1021/cr068123a
2. [2]
  (2) Koschwanez, H. E.; Reichert,W. M. Biomaterials 2007, 28,3687. doi: 10.1016/j.biomaterials.2007.03.034(2) Koschwanez, H. E.; Reichert,W. M. Biomaterials 2007, 28,3687. doi: 10.1016/j.biomaterials.2007.03.034
3. [3]
  (3) Hu, J. Biosens. Bioelectron. 2009, 24, 1083. doi: 10.1016/j.bios.2008.08.051(3) Hu, J. Biosens. Bioelectron. 2009, 24, 1083. doi: 10.1016/j.bios.2008.08.051
4. [4]
  (4) Ge, F.; Cao, R. G.; Zhu, B.; Li, J. J.; Xu, D. S. Acta Phys. -Chim. Sin. 2010, 26, 1779. [戈芳, 曹瑞国, 朱斌, 李经建, 徐东升. 物理化学学报, 2010, 26, 1779.] doi: 10.3866/PKU.WHXB20100736(4) Ge, F.; Cao, R. G.; Zhu, B.; Li, J. J.; Xu, D. S. Acta Phys. -Chim. Sin. 2010, 26, 1779. [戈芳, 曹瑞国, 朱斌, 李经建, 徐东升. 物理化学学报, 2010, 26, 1779.] doi: 10.3866/PKU.WHXB20100736
5. [5]
  (5) Willner, I.; Baron, R.;Willner, B. Biosens. Bioelectron. 2007,22, 1841. doi: 10.1016/j.bios.2006.09.018(5) Willner, I.; Baron, R.;Willner, B. Biosens. Bioelectron. 2007,22, 1841. doi: 10.1016/j.bios.2006.09.018
6. [6]
  (6) Zar osh, K.; Chaichi, M. J.; Shamsipur, M.; Hossienkhani, S.;Asghari, S.; Qandalee, M. Talanta 2012, 93, 37. doi: 10.1016/j.talanta.2011.11.029(6) Zar osh, K.; Chaichi, M. J.; Shamsipur, M.; Hossienkhani, S.;Asghari, S.; Qandalee, M. Talanta 2012, 93, 37. doi: 10.1016/j.talanta.2011.11.029
7. [7]
  (7) Xiao, X.; Zhou, B.; Zhu, L.; Xu, L.; Tan, L.; Tang, H.; Zhang,Y.; Xie, Q.; Yao, S. Sens. Actuators B 2012, 165, 126. doi: 10.1016/j.snb.2012.02.029(7) Xiao, X.; Zhou, B.; Zhu, L.; Xu, L.; Tan, L.; Tang, H.; Zhang,Y.; Xie, Q.; Yao, S. Sens. Actuators B 2012, 165, 126. doi: 10.1016/j.snb.2012.02.029
8. [8]
  (8) Willner, I.;Willner, B.; Katz, E. Bioelectrochemistry 2007, 70,2. doi: 10.1016/j.bioelechem.2006.03.013(8) Willner, I.;Willner, B.; Katz, E. Bioelectrochemistry 2007, 70,2. doi: 10.1016/j.bioelechem.2006.03.013
9. [9]
  (9) Heller, A.; Feldman, B. Chem. Rev. 2008, 108, 2482. doi: 10.1021/cr068069y(9) Heller, A.; Feldman, B. Chem. Rev. 2008, 108, 2482. doi: 10.1021/cr068069y
10. [10]
  (10) Guo, X. L.; Guo, M.;Wang, X. D. Acta Phys. -Chim. Sin. 2007,23, 585. [郭小丽, 郭敏, 王新东. 物理化学学报, 2007, 23,585.] doi: 10.3866/PKU.WHXB20070426(10) Guo, X. L.; Guo, M.;Wang, X. D. Acta Phys. -Chim. Sin. 2007,23, 585. [郭小丽, 郭敏, 王新东. 物理化学学报, 2007, 23,585.] doi: 10.3866/PKU.WHXB20070426
11. [11]
  (11) Cui, G.; Kim, S. J.; Choi, S. H.; Nam, H.; Cha, G. S.; Paeng, K.J. Anal. Chem. 2000, 72, 1925. doi: 10.1021/ac991213d(11) Cui, G.; Kim, S. J.; Choi, S. H.; Nam, H.; Cha, G. S.; Paeng, K.J. Anal. Chem. 2000, 72, 1925. doi: 10.1021/ac991213d
12. [12]
  (12) Weibel, M. K.; Bright, H. J. J. Biol. Chem. 1971, 246, 2743.(12) Weibel, M. K.; Bright, H. J. J. Biol. Chem. 1971, 246, 2743.
13. [13]
  (13) Wrighton, M. S. Science 1986, 231, 32. doi: 10.1126/science.231.4733.32(13) Wrighton, M. S. Science 1986, 231, 32. doi: 10.1126/science.231.4733.32
14. [14]
  (14) Frederick, K. R.; Tung, J.; Emerick, R. S.; Masiarz, F. R.;Chamberlain, S. H.; Vasavada, A.; Rosenberg, S.; Chakraborty,S.; Schopter, L. M.; Massey, V. J. Biol. Chem. 1990, 265, 3793.(14) Frederick, K. R.; Tung, J.; Emerick, R. S.; Masiarz, F. R.;Chamberlain, S. H.; Vasavada, A.; Rosenberg, S.; Chakraborty,S.; Schopter, L. M.; Massey, V. J. Biol. Chem. 1990, 265, 3793.
15. [15]
  (15) Hecht, H. J.; Schomburg, D.; Kalisz, H.; Schmid, R. D. Biosens. Bioelectron. 1993, 8, 197. doi: 10.1016/0956-5663(93)85033-K(15) Hecht, H. J.; Schomburg, D.; Kalisz, H.; Schmid, R. D. Biosens. Bioelectron. 1993, 8, 197. doi: 10.1016/0956-5663(93)85033-K
16. [16]
  (16) Yu, J.; Yu, D.; Zhao, T.; Zeng, B. Talanta 2008, 74, 1586. doi: 10.1016/j.talanta.2007.10.005(16) Yu, J.; Yu, D.; Zhao, T.; Zeng, B. Talanta 2008, 74, 1586. doi: 10.1016/j.talanta.2007.10.005
17. [17]
  (17) Deng, S.; Jian, G.; Lei, J.; Hu, Z.; Ju, H. Biosens. Bioelectron.2009, 25, 373. doi: 10.1016/j.bios.2009.07.016(17) Deng, S.; Jian, G.; Lei, J.; Hu, Z.; Ju, H. Biosens. Bioelectron.2009, 25, 373. doi: 10.1016/j.bios.2009.07.016
18. [18]
  (18) Wei, Y.; Li, Y.; Liu, X.; Xian, Y.; Shi, G.; Jin, L. Biosens. Bioelectron. 2010, 26, 275. doi: 10.1016/j.bios.2010.06.006(18) Wei, Y.; Li, Y.; Liu, X.; Xian, Y.; Shi, G.; Jin, L. Biosens. Bioelectron. 2010, 26, 275. doi: 10.1016/j.bios.2010.06.006
19. [19]
  (19) Zhang, G. L.; Pan, X. H.; Kan, J. Q.; Zhang, J. H.; Li, Y. F. Acta Phys. -Chim. Sin. 2003, 19, 533. [张国林, 潘献华, 阚锦晴,张景辉, 李永舫. 物理化学学报, 2003, 19, 533.] doi: 10.3866/PKU.WHXB20030611(19) Zhang, G. L.; Pan, X. H.; Kan, J. Q.; Zhang, J. H.; Li, Y. F. Acta Phys. -Chim. Sin. 2003, 19, 533. [张国林, 潘献华, 阚锦晴,张景辉, 李永舫. 物理化学学报, 2003, 19, 533.] doi: 10.3866/PKU.WHXB20030611
20. [20]
  (20) Qiu, J. D.; Huang, J.; Liang, R. P. Sens. Actuators B 2011, 160,287. doi: 10.1016/j.snb.2011.07.049(20) Qiu, J. D.; Huang, J.; Liang, R. P. Sens. Actuators B 2011, 160,287. doi: 10.1016/j.snb.2011.07.049
21. [21]
  (21) Chen, X.; Zhu, J.; Chen, Z.; Xu, C.;Wang, Y.; Yao, C. Sens. Actuators B 2011, 159, 220. doi: 10.1016/j.snb.2011.06.076(21) Chen, X.; Zhu, J.; Chen, Z.; Xu, C.;Wang, Y.; Yao, C. Sens. Actuators B 2011, 159, 220. doi: 10.1016/j.snb.2011.06.076
22. [22]
  (22) Che, X.; Yuan, R.; Chai, Y.; Li, J.; Song, Z.; Li,W.; Zhong, X.Colloids Surf. B 2011, 84, 454. doi: 10.1016/j.colsurfb.2011.01.041(22) Che, X.; Yuan, R.; Chai, Y.; Li, J.; Song, Z.; Li,W.; Zhong, X.Colloids Surf. B 2011, 84, 454. doi: 10.1016/j.colsurfb.2011.01.041
23. [23]
  (23) Valstar, A.; Vasilescu, M.; Vi uroux, C.; Stilbs, P.; Almgren,M. Langmuir 2001, 17, 3208. doi: 10.1021/la0016221(23) Valstar, A.; Vasilescu, M.; Vi uroux, C.; Stilbs, P.; Almgren,M. Langmuir 2001, 17, 3208. doi: 10.1021/la0016221
24. [24]
  (24) Rodahl, M.; Höök, F.; Krozer, A.; Kasemo, B.; Breszinsky, P.Rev. Sci. Instrum. 1995, 66, 3924. doi: 10.1063/1.1145396(24) Rodahl, M.; Höök, F.; Krozer, A.; Kasemo, B.; Breszinsky, P.Rev. Sci. Instrum. 1995, 66, 3924. doi: 10.1063/1.1145396
25. [25]
  (25) Voinova, M. V.; Rodahl, M.; Jonson, M.; Kasemo, B. Phys. Scrip. 1999, 59, 31.(25) Voinova, M. V.; Rodahl, M.; Jonson, M.; Kasemo, B. Phys. Scrip. 1999, 59, 31.
26. [26]
  (26) Bloomfield, V. Biochemistry 1965, 5, 684.(26) Bloomfield, V. Biochemistry 1965, 5, 684.
27. [27]
  (27) Hirayama, K.; Akashi, S.; Furuya, M.; Fukuhara, K. L.Biochem. Biophys. Res. Commun. 1990, 173, 639. doi: 10.1016/S0006-291X(05)80083-X(27) Hirayama, K.; Akashi, S.; Furuya, M.; Fukuhara, K. L.Biochem. Biophys. Res. Commun. 1990, 173, 639. doi: 10.1016/S0006-291X(05)80083-X
28. [28]
  (28) Bos, O. J. M.; Labro, J. F. A.; Fischer, M. J. E.;Wilting, J.;Janssen, L. H. M. J. Biol. Chem. 1989, 264, 953.(28) Bos, O. J. M.; Labro, J. F. A.; Fischer, M. J. E.;Wilting, J.;Janssen, L. H. M. J. Biol. Chem. 1989, 264, 953.
29. [29]
  (29) Bontempo, D.; Heredia, K. L.; Fish, B. A.; Maynard, H. D.J. Am. Chem. Soc. 2004, 126, 15372. doi: 10.1021/ja045063m(29) Bontempo, D.; Heredia, K. L.; Fish, B. A.; Maynard, H. D.J. Am. Chem. Soc. 2004, 126, 15372. doi: 10.1021/ja045063m
30. [30]
  (30) Heredia, K. L.; Bontempo, D.; Ly, T.; Byers, J. T.; Halstenberg,S.; Maynard, H. D. J. Am. Chem. Soc. 2005, 127, 16955. doi: 10.1021/ja054482w(30) Heredia, K. L.; Bontempo, D.; Ly, T.; Byers, J. T.; Halstenberg,S.; Maynard, H. D. J. Am. Chem. Soc. 2005, 127, 16955. doi: 10.1021/ja054482w
31. [31]
  (31) Liu, J.; Bulmus, V.; Herlambang, D. L.; Barner-Kowollik, C.;Stenzel, M. H.; Davis, T. P. Angew. Chem. Int. Edit. 2007, 46,3099.(31) Liu, J.; Bulmus, V.; Herlambang, D. L.; Barner-Kowollik, C.;Stenzel, M. H.; Davis, T. P. Angew. Chem. Int. Edit. 2007, 46,3099.
32. [32]
  (32) Boyer, C.; Bulmus, V.; Liu, J.; Davis, T. P.; Stenzel, M. H.;Barner-Kowollik, C. J. Am. Chem. Soc. 2007, 129, 7145. doi: 10.1021/ja070956a(32) Boyer, C.; Bulmus, V.; Liu, J.; Davis, T. P.; Stenzel, M. H.;Barner-Kowollik, C. J. Am. Chem. Soc. 2007, 129, 7145. doi: 10.1021/ja070956a
33. [33]
  (33) Cascâo Pereira, L. G.; Théodoly, O.; Blanch, H.W.; Radke, C. J.Langmuir 2003, 19, 2349. doi: 10.1021/la020720e(33) Cascâo Pereira, L. G.; Théodoly, O.; Blanch, H.W.; Radke, C. J.Langmuir 2003, 19, 2349. doi: 10.1021/la020720e
34. [34]
  (34) Zhu,W.; Kapteijn, F.; Moulijn, J. A.; den Exter, M. C.; Jansen,J. C. Langmuir 2000, 16, 3322. doi: 10.1021/la9914007(34) Zhu,W.; Kapteijn, F.; Moulijn, J. A.; den Exter, M. C.; Jansen,J. C. Langmuir 2000, 16, 3322. doi: 10.1021/la9914007
35. [35]
  (35) Wassell, D. T.; Hall, R. C.; Embery, G. Biomaterials 1995, 16,697. doi: 10.1016/0142-9612(95)99697-K(35) Wassell, D. T.; Hall, R. C.; Embery, G. Biomaterials 1995, 16,697. doi: 10.1016/0142-9612(95)99697-K
36. [36]
  (36) Rabe, M.; Verdes, D.; Zimmermann, J.; Seeger, S. J. Phys. Chem. B 2008, 112, 13971. doi: 10.1021/jp804532v(36) Rabe, M.; Verdes, D.; Zimmermann, J.; Seeger, S. J. Phys. Chem. B 2008, 112, 13971. doi: 10.1021/jp804532v
37. [37]
  (37) Zhu, H.; Srivastava, R.; Brown, J. Q.; McShane, M. J.Bioconjugate Chem. 2005, 16, 1451. doi: 10.1021/bc050171z(37) Zhu, H.; Srivastava, R.; Brown, J. Q.; McShane, M. J.Bioconjugate Chem. 2005, 16, 1451. doi: 10.1021/bc050171z
38. [38]
  (38) Shu, F. R.;Wilson, G. S. Anal. Chem. 1976, 48, 1679. doi: 10.1021/ac50006a014(38) Shu, F. R.;Wilson, G. S. Anal. Chem. 1976, 48, 1679. doi: 10.1021/ac50006a014
39. [39]
  (39) Kamin, R. A.;Wilson, G. S. Anal. Chem. 1980, 52, 1198. doi: 10.1021/ac50058a010
  (39) Kamin, R. A.;Wilson, G. S. Anal. Chem. 1980, 52, 1198. doi: 10.1021/ac50058a010

扫一扫看文章

计量

PDF下载量: 933
文章访问数: 3500
HTML全文浏览量: 79

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

聚甲基丙烯酸甲酯-牛血清白蛋白核壳纳米粒子在金表面的吸附过程及在传感器中的应用

English

Adsorption of Core-Shell Poly(Methyl Methacrylate)-Bovine Serum Albumin Nanoparticles on ld Surface and Its Sensor Application

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

聚甲基丙烯酸甲酯-牛血清白蛋白核壳纳米粒子在金表面的吸附过程及在传感器中的应用

English

Adsorption of Core-Shell Poly(Methyl Methacrylate)-Bovine Serum Albumin Nanoparticles on ld Surface and Its Sensor Application

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs