基于光波导分光光谱技术研究蛋白质与亚甲基蓝的竞争吸附行为

引用本文: 邓琳, 祁志美. 基于光波导分光光谱技术研究蛋白质与亚甲基蓝的竞争吸附行为[J]. 物理化学学报, 2010, 26(10): 2672-2678. doi: 10.3866/PKU.WHXB20101003 shu

Citation: DENG Lin, QI Zhi-Mei. Study of Competitive Adsorption Behavior of Protein andMethylene Blue by Optical Waveguide Spectroscopy[J]. Acta Physico-Chimica Sinica, 2010, 26(10): 2672-2678. doi: 10.3866/PKU.WHXB20101003 shu

基于光波导分光光谱技术研究蛋白质与亚甲基蓝的竞争吸附行为

邓琳 ,
祁志美

基金项目:
国家重点基础研究发展计划(973)(2009CB320300) (973)(2009CB320300)

国家自然科学基金(60978042) (60978042)

中科院&ldquo

百人计划&rdquo

择优支持项目资助

摘要:

通过利用时间分辨光波导分光光谱技术原位测量从蛋白质-亚甲基蓝(MB) 混合水溶液吸附到亲水玻璃光波导表面的MB 可见光吸收谱, 观测到在溶液pH 值低于蛋白质等电点时MB 与牛血清蛋白(BSA) 以及MB 与血红蛋白(Hb) 存在竞争吸附行为, 进一步测得这种竞争吸附行为对蛋白质浓度十分敏感, 可以用于简单测定溶液中的蛋白质含量. 基于Langmuir 等温吸附理论推导出了两种分子竞争吸附的动力学方程, 并利用该动力学方程对实验测得的吸光度随时间变化曲线进行了最佳拟合, 揭示了玻璃表面吸附的MB 分子个数在达到最大值后随时间呈指数衰减, 同时得出拟合参数与蛋白质浓度呈准线性关系.

关键词:

English

Study of Competitive Adsorption Behavior of Protein andMethylene Blue by Optical Waveguide Spectroscopy

DENG Lin ,
QI Zhi-Mei

1.
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, P. R. China
Received Date: 24 May 2010
Available Online: 27 September 2010
Fund Project:
国家重点基础研究发展计划(973)(2009CB320300)

国家自然科学基金(60978042)

中科院&ldquo

百人计划&rdquo

择优支持项目资助

Abstract:

We used time-resolved optical waveguide spectroscopy (OWGS) to in-situ measure visible absorption spectrum for methylene blue (MB) adsorbed ona hydrophilic glass waveguide from an aqueous solution containing proteins such as bovine serum albumin (BSA) and hemoglobin (Hb). The competitive adsorption of MB and the protein was detected at a solution pH lower than the isoelectric point of the protein. Since the competitive adsorption behavior of MB and the protein is very sensitive to the protein concentration, the protein content in the mixed solution was readily determined by time-resolved OWGS. In addition, a kinetic equation for the competitive adsorption of the two molecules was deduced based on the Langmuir adsorption isotherm. The best fit of the measured time course of the absorbance with the theoretical kinetic equation reveals that the number of MB molecules adsorbed on the surface exponentially decreases with time after reaching a maximum.A quasi-linear relationship between the fitting parameters and the protein concentration was also obtained.

Key words:

1. [1]
  
  1. Marco, A.; Rubio, R.; Compano, R.; Casals, I. Talanta, 2002, 57: 1019
  1. Marco, A.; Rubio, R.; Compano, R.; Casals, I. Talanta, 2002, 57: 1019
2. [2]
  2. Coakley, W. T.; James, C. J. Anal. Biochem., 1978, 85: 902. Coakley, W. T.; James, C. J. Anal. Biochem., 1978, 85: 90
3. [3]
  3. Shen, W.; Chen, J. Z.; Dai, C. J. Food Res. Dev., 2009, 30: 96 [沈文,陈均志,代春吉. 食品研究与开发, 2009, 30: 96]3. Shen, W.; Chen, J. Z.; Dai, C. J. Food Res. Dev., 2009, 30: 96 [沈文,陈均志,代春吉. 食品研究与开发, 2009, 30: 96]
4. [4]
  4. Li, J.; Zhang, Y. T.; Zeng, W.; Luo, X.; Liao, C. C. Chin. J. Biol., 2000, 13: 118 [李娟,张耀庭,曾伟,罗璇,廖长春.中国生物制品学杂志, 2000, 13: 118]4. Li, J.; Zhang, Y. T.; Zeng, W.; Luo, X.; Liao, C. C. Chin. J. Biol., 2000, 13: 118 [李娟,张耀庭,曾伟,罗璇,廖长春.中国生物制品学杂志, 2000, 13: 118]
5. [5]
  5. Minagawa, M.; Winter, D. A.; Kaplan, I. R. Anal. Chem., 1984, 56: 18595. Minagawa, M.; Winter, D. A.; Kaplan, I. R. Anal. Chem., 1984, 56: 1859
6. [6]
  6. Penninckx,W.; Verbeke, J. S.; Vankeerberghen, P.; Massart, D. L. Anal. Chem., 1996, 68: 4816. Penninckx,W.; Verbeke, J. S.; Vankeerberghen, P.; Massart, D. L. Anal. Chem., 1996, 68: 481
7. [7]
  7. Yu, X. L.; Wei, X.; Wang, D. X.; Ding, X.; Liao, W.; Zhao, X. S. Acta Phys.-Chim. Sin., 2005, 21: 888 [余兴龙,魏星,王鼎新, 定翔,廖伟,赵新生.物理化学学报, 2005, 21: 888]7. Yu, X. L.; Wei, X.; Wang, D. X.; Ding, X.; Liao, W.; Zhao, X. S. Acta Phys.-Chim. Sin., 2005, 21: 888 [余兴龙,魏星,王鼎新, 定翔,廖伟,赵新生.物理化学学报, 2005, 21: 888]
8. [8]
  8. Schmitt, K.; Schirmer, B.; Hoffmann, C.; Brandenburg, A.; Meyrueis, P. Biosens. Bioelectro., 2007, 22: 25918. Schmitt, K.; Schirmer, B.; Hoffmann, C.; Brandenburg, A.; Meyrueis, P. Biosens. Bioelectro., 2007, 22: 2591
9. [9]
  9. Wu, Z.W.; Liu, Q. J.; Wu, L. W.; Xie, X.; Zhuang, Y.;Wen, T.; Bai, Y. F.; Lu, Z. H. Acta Optica Sinica, 2007, 27: 521 [吴中伟, 刘全俊,吴凌伟, 谢骁,庄萤,温恬, 白云飞, 陆祖宏.光学学报, 2007, 27: 521]9. Wu, Z.W.; Liu, Q. J.; Wu, L. W.; Xie, X.; Zhuang, Y.;Wen, T.; Bai, Y. F.; Lu, Z. H. Acta Optica Sinica, 2007, 27: 521 [吴中伟, 刘全俊,吴凌伟, 谢骁,庄萤,温恬, 白云飞, 陆祖宏.光学学报, 2007, 27: 521]
10. [10]
  10. Kim, N.; Kim, D. K.; Kim, W. Y. Food Chem., 2008, 108: 76810. Kim, N.; Kim, D. K.; Kim, W. Y. Food Chem., 2008, 108: 768
11. [11]
  11. Enami, Y.; Fukuda, T.; Suye, S. Appl. Phys. Lett., 2007, 91: 203507-111. Enami, Y.; Fukuda, T.; Suye, S. Appl. Phys. Lett., 2007, 91: 203507-1
12. [12]
  12. Kim, N.; Kim, D. Y.; Cho, Y. J.; Moon, D. K.; Kim, W. Y. Biosens. Bioelectro., 2008, 24: 39112. Kim, N.; Kim, D. Y.; Cho, Y. J.; Moon, D. K.; Kim, W. Y. Biosens. Bioelectro., 2008, 24: 391
13. [13]
  13. Choquette, S. J.; Browm, L. L.; Durst, R. A. Anal. Chem., 1992, 64: 5513. Choquette, S. J.; Browm, L. L.; Durst, R. A. Anal. Chem., 1992, 64: 55
14. [14]
  14. Bradshaw, J. T.; Mendes, S. B.; Saavedra, S. S. Anal. Chem., 2005: 29A14. Bradshaw, J. T.; Mendes, S. B.; Saavedra, S. S. Anal. Chem., 2005: 29A
15. [15]
  15. Qi, Z. M.; Matsuda, N.; Takatsu, A.; Kato, K. J. Phys. Chem. B, 2003, 107: 687315. Qi, Z. M.; Matsuda, N.; Takatsu, A.; Kato, K. J. Phys. Chem. B, 2003, 107: 6873
16. [16]
  16. Cameron, P. J.; Jenkins, A. T. A.; Knoll, W.; Marken, F.; Milsom, E. V.; Williams, T. L. J. Mater. Chem., 2008, 18: 430416. Cameron, P. J.; Jenkins, A. T. A.; Knoll, W.; Marken, F.; Milsom, E. V.; Williams, T. L. J. Mater. Chem., 2008, 18: 4304
17. [17]
  17. Qi, Z. M.; Xia, S. H.; Matsuda, N. Anal. Biochem., 2008, 374: 19617. Qi, Z. M.; Xia, S. H.; Matsuda, N. Anal. Biochem., 2008, 374: 196
18. [18]
  18. Mendes, S. B.; Saavedra, S. S. Opt. Express, 1999, 4: 44918. Mendes, S. B.; Saavedra, S. S. Opt. Express, 1999, 4: 449
19. [19]
  19. Fujita, K.; Taniguchi, K.; Ohno, H. Talanta, 2005, 65: 106619. Fujita, K.; Taniguchi, K.; Ohno, H. Talanta, 2005, 65: 1066
20. [20]
  20. Deng, L.; Lu, D. F.; Qi, Z. M. Acta Phys.-Chim. Sin., 2009, 25: 2481 [邓琳,逯丹凤,祁志美. 物理化学学报, 2009, 25: 2481]20. Deng, L.; Lu, D. F.; Qi, Z. M. Acta Phys.-Chim. Sin., 2009, 25: 2481 [邓琳,逯丹凤,祁志美. 物理化学学报, 2009, 25: 2481]
21. [21]
  21. Azizian, S.; Haerifar, M.; Parsa, J. B. Chemosphere, 2007, 68: 204021. Azizian, S.; Haerifar, M.; Parsa, J. B. Chemosphere, 2007, 68: 2040
22. [22]
  22. mri, S.; Seguin, J. L.; Guerin, J.; Aguir, K. Sens. Actuators, 2006, 114: 45122. mri, S.; Seguin, J. L.; Guerin, J.; Aguir, K. Sens. Actuators, 2006, 114: 451
23. [23]
  23. Koutsopoulos, S.; Patzsch, K.; Bosker,W. T. E.; Norde, W. Langmuir, 2007, 23: 200023. Koutsopoulos, S.; Patzsch, K.; Bosker,W. T. E.; Norde, W. Langmuir, 2007, 23: 2000
24. [24]
  24. Kusakari, A.; Izumi, M.; Ohnuki, H. Colloids Surf. A, 2008, 321: 4724. Kusakari, A.; Izumi, M.; Ohnuki, H. Colloids Surf. A, 2008, 321: 47
25. [25]
  25. Can, O.; Holland, N. B. J. Colloid Interface Sci., 2009, 329: 24
  25. Can, O.; Holland, N. B. J. Colloid Interface Sci., 2009, 329: 24

扫一扫看文章

计量

PDF下载量: 1271
文章访问数: 3508
HTML全文浏览量: 62

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于光波导分光光谱技术研究蛋白质与亚甲基蓝的竞争吸附行为

English

Study of Competitive Adsorption Behavior of Protein andMethylene Blue by Optical Waveguide Spectroscopy

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于光波导分光光谱技术研究蛋白质与亚甲基蓝的竞争吸附行为

English

Study of Competitive Adsorption Behavior of Protein andMethylene Blue by Optical Waveguide Spectroscopy

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs