引用本文:
赵海燕, 宋时英, 林政炯, 杜晓燕, 周元聪. pBPB共价修饰蝮蛇酸性磷脂酶A2的晶体学数据[J]. 物理化学学报,
1997, 13(05): 449-451.
doi:
10.3866/PKU.WHXB19970512
Citation: Zhao Hai-Yan, Song Shi-Ying, Lin Zheng-Jiong, Du Xiao-Yan, Zhou Yuan-Cong. The Crystallization of Acidic Phospholipase A2 from the Venom of Agkistrodon Blomhoffii Brevicaudus Covalently Inhibited by p-Bromo-phenacyl-bromide[J]. Acta Physico-Chimica Sinica, 1997, 13(05): 449-451. doi: 10.3866/PKU.WHXB19970512
Citation: Zhao Hai-Yan, Song Shi-Ying, Lin Zheng-Jiong, Du Xiao-Yan, Zhou Yuan-Cong. The Crystallization of Acidic Phospholipase A2 from the Venom of Agkistrodon Blomhoffii Brevicaudus Covalently Inhibited by p-Bromo-phenacyl-bromide[J]. Acta Physico-Chimica Sinica, 1997, 13(05): 449-451. doi: 10.3866/PKU.WHXB19970512
English
The Crystallization of Acidic Phospholipase A2 from the Venom of Agkistrodon Blomhoffii Brevicaudus Covalently Inhibited by p-Bromo-phenacyl-bromide
Abstract:
The Crystals of acidic phospholipase A2 from the venom of Agkistrodon blomhoffii brevicaudus (i.e. Agkistrodon halys pallas) covalently modified with p-bromo-phenacylbromide were obtained by the method of hanging drop vapor diffusion. Crystallization droplet was composed of 0.06mol?L-1 Na(CH3)2AsO2 (pH=6.5), 24.8% (v/v) 1,4-butanediol, and 4.5mg?mL-1 protein. The crystal data are of a=b=82.82?., c=32.85?, space group P61. 8945 unique reflections with 1.93 ? resolution were measured on a Siemens area detector X-ray diffractometor, of which 8069 reflections having F0 >2σ(F). The experimental results show that the crystals are suitable to a structure analysis of high resolution.
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Key words:
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Phospolipase A2
- / pBPB inhibition
- / Crystallization
- / X-ray diffraction
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