纳米粒子与细胞相互作用的力学

引用本文: 展金秀, 冯峰, 许敏, 姚立, 葛茂发. 纳米粒子与细胞相互作用的力学–化学偶联研究进展[J]. 物理化学学报, 2020, 36(1): 190507. doi: 10.3866/PKU.WHXB201905076 shu

Citation: Zhan Jinxiu, Feng Feng, Xu Ming, Yao Li, Ge Maofa. Progress in Chemo–Mechanical Interactions between Nanoparticles and Cells[J]. Acta Physico-Chimica Sinica, 2020, 36(1): 190507. doi: 10.3866/PKU.WHXB201905076 shu

纳米粒子与细胞相互作用的力学–化学偶联研究进展

展金秀 ^1,2, ,
冯峰 ^1,2, ,
许敏 ^1,2, ,
姚立 ^{1,2,
,} ,
葛茂发 ^{1,2,
,}

1.
中国科学院化学研究所，北京分子科学国家研究中心，分子动态与稳态结构实验室，中国科学院分子科学科教融合卓越创新中心，北京 100190
2.
中国科学院大学，北京 100049

作者简介:

姚立，出生于1979年。毕业于中国科学院化学研究所，获理学博士学位。中国科学院化学研究所研究员，博士生导师。长期从事分子细胞相互作用与纳米生物技术研究;
葛茂发，出生于1970年。毕业于吉林大学，获理学博士学位。中国科学院化学研究所研究员，博士生导师。研究方向包括大气化学、环境化学、环境催化、纳米材料和污染控制技术;

通讯作者: 姚立, yaoli@iccas.ac.cn; 葛茂发, gemaofa@iccas.ac.cn

基金项目:

国家自然科学基金(21778055, 21573250)，国家重点研发计划(2018YFA0208800)和北京市自然科学基金(L172048)资助项目

摘要: 纳米粒子在生物医学和大气环境领域的广泛研究使得其生物安全性越来越受到重视。目前已经有许多研究关注纳米粒子与细胞的相互作用及细胞毒性问题。本综述从细胞力学-化学偶联的角度总结了近五年来有关纳米粒子与细胞相互作用的研究进展。首先介绍了与细胞力学-化学偶联性质相关的分子基础以及目前检测细胞机械性质的纳米技术，然后重点讨论了纳米粒子对细胞粘附、骨架、刚度和迁移性质的影响。在此基础上，进一步指出了纳米生物力学-化学偶联的挑战与展望。

关键词:

English

Progress in Chemo–Mechanical Interactions between Nanoparticles and Cells

Zhan Jinxiu ^1,2, ,
Feng Feng ^1,2, ,
Xu Ming ^1,2, ,
Yao Li ^{1,2,
,} ,
Ge Maofa ^{1,2,
,}

1.
Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China
2.
University of Chinese Academy of Sciences, Beijing 100049, P. R. China

Corresponding author: Emails: yaoli@iccas.ac.cn(L. Y.); Tel.: +86-10-62588930(L. Y.); Emails: gemaofa@iccas.ac.cn(M. G.)

Received Date: 28 May 2019
Accepted Date: 08 July 2019
Revised Date: 05 July 2019
Available Online: 15 January 2020
Fund Project:

The project was supported by the National Natural Science Foundation of China (21778055, 21573250), the National Key Research and Development Program of China (2018YFA0208800), and the Beijing Natural Science Foundation, China (L172048)

Abstract: The biosafety of nanoparticles is gaining extensive attention due to their dichotomous effects in fields of biomedicine and atmospheric chemistry. A number of studies have been carried out focusing on the cytotoxicity of nanoparticles and their interactions with cells. However, the mechanism of nanoparticle–cell interactions remains unclear. Here, we review the latest progress in the study of nanoparticle-cell interactions from a cellular chemo-mechanical perspective. Cell mechanics play an important role in cell differentiation, proliferation, apoptosis, polarization, adhesion, and migration. An understanding of the effects of nanoparticles on cell mechanics is therefore needed in order to enhance comprehension of nanoparticle–cell interactions. Firstly, the main molecules and signal pathways related to mechanical chemistry are introduced from three perspectives: cell surface adhesion receptors, the cytoskeleton, and the nucleus. Specifically, integrins and cadherins play a critical role in sensing both the external mechanical force and the force of cell transmission. Actin and microtubules, which are two components of the cytoskeletal network, act as a bridge in mechanical conduction. The nucleus can also be mechanically stressed by the surrounding cytoskeleton through the contraction of the matrix. The nuclear envelope also plays important roles in sensing mechanical signals and in adjusting the morphology and function of the nucleus. We summarize the major nanoparticle-based tools used in the laboratory for the study of cell mechanics, which includes traction force microscopy, atomic force microscopy, optical tweezers, magnetic manipulation, micropillars, and force-induced remnant magnetization spectroscopy. In addition, we discuss the effects that nanoparticles have on cell mechanics. Nanoparticles interact with the adhesion of molecules on the cell membrane surface and on cell cytoskeletal proteins, which further affects the mechanical properties involved in cell stiffness, cell adhesion, and cell migration. Overall, the general conclusions regarding the effects of nanoparticles on cell mechanics are as follows: (1) Nanoparticles can affect cell adhesion by disrupting tight and adherent junctions, and by regulating cell-extracellular matrix adhesion; (2) Nanoparticles can interact with cytoskeletal proteins (actins and tubulins) leading to structural reorganization or disruption of microtubules and F-actin; (3) Cell stiffness changes with the structural reorganization of the cytoskeleton; (4) Cell migration ability can be affected through changes in the cytoskeleton, cell adhesion, and the expression of cell migration-related proteins/molecules. To develop the nano-biosafety evaluation system, future studies should attempt to gain a better understanding of the molecular mechanisms involved with regards to nanoparticles and cell mechanics. Ultimately, further development of new methods and technologies based on nano-mechanical chemistry for diagnosis and treatment purposes are expected, given the wide application of nanomaterials in the biomedical field.

Key words:

1. [1]
  Grabinski, C.; Schaeublin, N.; Wijaya, A.; D'Couto, H.; Baxamusa, S. H.; Hamad-Schifferli, K.; Hussain, S. M. ACS Nano 2011, 5 (4), 2870. doi: 10.1021/nn103476x
2. [2]
  Pati, R.; Das, I.; Mehta, R. K.; Sahu, R.; Sonawane, A. Toxicol. Sci. 2016, 150 (2), 454. doi: 10.1093/toxsci/kfw010
3. [3]
  Li, J. C.; Mao, H. L.; Kawazoe, N.; Chen, G. P. Biomater. Sci. 2017, 5 (2), 173. doi: 10.1039/c6bm00714g
4. [4]
  Rotsch, C.; Radmacher, M. Biophys. J. 2000, 78 (1), 520. doi: 10.1016/s0006-3495(00)76614-8
5. [5]
  Tay, C. Y.; Cai, P. Q.; Setyawati, M. I.; Fang, W. R.; Tan, L. P.; Hong, C. H. L.; Chen, X. D.; Leong, D. T. Nano Lett. 2014, 14 (1), 83. doi: 10.1021/nl4032549
6. [6]
  Li, Y.; Jing, L.; Yu, Y. B.; Yu, Y.; Duan, J. C.; Yang, M.; Geng, W. J.; Jiang, L. Z.; Li, Q. L.; Sun, Z. W. Part. Part. Syst. Charact. 2015, 32 (6), 636. doi: 10.1002/ppsc.201400180
7. [7]
  Nakayama, K. H.; Surya, V. N.; Gole, M.; Walker, T. W.; Yang, W. G.; Lai, E. S.; Ostrowski, M. A.; Fuller, G. G.; Dunn, A. R.; Huang, N. F. Nano Lett. 2016, 16 (1), 410. doi: 10.1021/acs.nanolett.5b04028
8. [8]
  Hynes, R. O. Cell 2002, 110 (6), 673. doi: 10.1016/s0092-8674(02)00971-6
9. [9]
  Guo, W. J.; Giancotti, F. G. Nat. Rev. Mol. Cell Biol. 2004, 5 (10), 816. doi: 10.1038/nrm1490
10. [10]
  Mitra, S. K.; Hanson, D. A.; Schlaepfer, D. D. Nat. Rev. Mol. Cell Biol. 2005, 6 (1), 56. doi: 10.1038/nrm1549
11. [11]
  Li, Z. H.; Lee, H. J.; Zhu, C. Exp. Cell Res. 2016, 349 (1), 85. doi: 10.1016/j.yexcr.2016.10.001
12. [12]
  Geiger, B.; Yamada, K. M. CSH Perspect. Biol. 2011, 3 (5), 21. doi: 10.1101/cshperspect.a005033
13. [13]
  Jiang, G. Y.; Giannone, G.; Critchley, D. R.; Fukumoto, E.; Sheetz, M. P. Nature 2003, 424 (6946), 334. doi: 10.1038/nature01805
14. [14]
  Kukkurainen, S.; Maatta, J. A.; Saeger, J.; Valjakka, J.; Vogel, V.; Hytonen, V. P. Mol. Biosyst. 2014, 10 (12), 3217. doi: 10.1039/c4mb00341a
15. [15]
  Yao, M. X.; Goult, B. T.; Chen, H.; Cong, P. W.; Sheetz, M. P.; Yan, J. Sci. Rep. 2014, 4, 7. doi: 10.1038/srep04610
16. [16]
  Mitra, S. K.; Schlaepfer, D. D. Curr. Opin. Cell Biol. 2006, 18 (5), 516. doi: 10.1016/j.ceb.2006.08.011
17. [17]
  Beningo, K. A.; Dembo, M.; Kaverina, I.; Small, J. V.; Wang, Y. L. J. Cell Biol. 2001, 153 (4), 881. doi: 10.1083/jcb.153.4.881
18. [18]
  Scarpa, E.; Szabo, A.; Bibonne, A.; Theveneau, E.; Parsons, M.; Mayor, R. Dev. Cell 2015, 34 (4), 421. doi: 10.1016/j.devcel.2015.06.012
19. [19]
  Yao, M. X.; Qiu, W.; Liu, R. C.; Efremov, A. K.; Cong, P. W.; Seddiki, R.; Payre, M.; Lim, C. T.; Ladoux, B.; Mege, R. M.; et al. Nat. Commun. 2014, 5, 11. doi: 10.1038/ncomms5525
20. [20]
  Mui, K. L.; Chen, C. S.; Assoian, R. K. J. Cell Sci. 2016, 129 (6), 1093. doi: 10.1242/jcs.183699
21. [21]
  Auernheimer, V.; Lautscham, L. A.; Leidenberger, M.; Friedrich, O.; Kappes, B.; Fabry, B.; Goldmann, W. H. J. Cell Sci. 2015, 128 (18), 3435. doi: 10.1242/jcs.172031
22. [22]
  Gasparski, A. N.; Beningo, K. A. Arch. Biochem. Biophys. 2015, 586, 20. doi: 10.1016/j.abb.2015.07.017
23. [23]
  Kiefel, H.; Bondong, S.; Hazin, J.; Ridinger, J.; Schirmer, U.; Riedle, S.; Altevogt, P. Cell Adhes. Migr. 2012, 6 (4), 374. doi: 10.4161/cam.20832
24. [24]
  Colombo, F.; Meldolesi, J. Trends Pharmacol. Sci. 2015, 36 (11), 769. doi: 10.1016/j.tips.2015.08.004
25. [25]
  Bershadsky, A.; Chausovsky, A.; Becker, E.; Lyubimova, A.; Geiger, B. Curr. Biol. 1996, 6 (10), 1279. doi: 10.1016/s0960-9822(02)70714-8
26. [26]
  Padmakumar, V. C.; Libotte, T.; Lu, W. S.; Zaim, H.; Abraham, S.; Noegel, A. A.; Gotzmann, J.; Foisner, R.; Karakesisoglou, L. J. Cell Sci. 2005, 118 (15), 3419. doi: 10.1242/jcs.02471
27. [27]
  Haque, F.; Lloyd, D. J.; Smallwood, D. T.; Dent, C. L.; Shanahan, C. M.; Fry, A. M.; Trembath, R. C.; Shackleton, S. Mol. Cell. Biol. 2006, 26 (10), 3738. doi: 10.1128/mcb.26.10.3738-3751.2006
28. [28]
  Luxton, G. W. G.; Gomes, E. R.; Folker, E. S.; Vintinner, E.; Gundersen, G. G. Science 2010, 329 (5994), 956. doi: 10.1126/science.1189072
29. [29]
  Guilluy, C.; Osborne, L. D.; Van Landeghem, L.; Sharek, L.; Superfine, R.; Garcia-Mata, R.; Burridge, K. Nat. Cell Biol. 2014, 16 (4), 376. doi: 10.1038/ncb2927
30. [30]
  Fanucchi, S.; Shibayama, Y.; Burd, S.; Weinberg, M. S.; Mhlanga, M. M. Cell 2013, 155 (3), 606. doi: 10.1016/j.cell.2013.09.051
31. [31]
  Dekker, J.; Mirny, L. Cell 2016, 164 (6), 1110. doi: 10.1016/j.cell.2016.02.007
32. [32]
  Uhler, C.; Shivashankar, G. V. Nat. Rev. Mol. Cell Biol. 2017, 18 (12), 717. doi: 10.1038/nrm.2017.101
33. [33]
  Lv, L. W.; Tang, Y. M.; Zhang, P.; Liu, Y. S.; Bai, X. S.; Zhou, Y. S. Tissue Eng. Part B-Rev. 2018, 24 (2), 112. doi: 10.1089/ten.teb.2017.0287
34. [34]
  Jaalouk, D. E.; Lammerding, J. Nat. Rev. Mol. Cell Biol. 2009, 10 (1), 63. doi: 10.1038/nrm2597
35. [35]
  Schwarz, U. S.; Soine, J. R. D. Biochim. Biophys. Acta-Mol. Cell Res. 2015, 1853 (11), 3095. doi: 10.1016/j.bbamcr.2015.05.028
36. [36]
  Muller, D. J.; Dufrene, Y. F. Trends Cell Biol. 2011, 21 (8), 461. doi: 10.1016/j.tcb.2011.04.008
37. [37]
  Ahmed, W. W.; Fodor, E.; Betz, T. Biochim. Biophys. Acta-Mol. Cell Res. 2015, 1853 (11), 3083. doi: 10.1016/j.bbamcr.2015.05.022
38. [38]
  Castillo, M.; Ebensperger, R.; Wirtz, D.; Walczak, M.; Hurtado, D. E.; Celedon, A. J. Biomed. Mater. Res. Part B 2014, 102 (8), 1779. doi: 10.1002/jbm.b.33167
39. [39]
  Berret, J. F. Nat. Commun. 2016, 7, 10134. doi: 10.1038/ncomms10134
40. [40]
  Etoc, F.; Lisse, D.; Bellaiche, Y.; Piehler, J.; Coppey, M.; Dahan, M. Nat. Nanotechnol. 2013, 8 (3), 193. doi: 10.1038/nnano.2013.23
41. [41]
  Tan, J. L.; Tien, J.; Pirone, D. M.; Gray, D. S.; Bhadriraju, K.; Chen, C. S. Pro. Nat. Acad. Sci. U.S.A. 2003, 100 (4), 1484. doi: 10.1073/pnas.0235407100
42. [42]
  Yao, L.; Xu, S. J. Angew. Chem.-Int. Edit. 2011, 50 (19), 4407. doi: 10.1002/anie.201007297
43. [43]
  Yao, L.; Li, Y.; Tsai, T. W.; Xu, S. J.; Wang, Y. H. Angew. Chem. Int. Ed. 2013, 52 (52), 14041. doi: 10.1002/anie.201307419
44. [44]
  Yao, L.; Xu, S. J. J. Phys. Chem. B 2012, 116 (33), 9944. doi: 10.1021/jp304335a
45. [45]
  Zhang, D.; Feng, F.; Li, Q. L.; Wang, X. Y.; Yao, L. Biomaterials 2018, 173, 22. doi: 10.1016/j.biomaterials.2018.04.045
46. [46]
  Yu, C.; Zhang, D.; Feng, X.; Chai, Y.; Lu, P.; Li, Q.; Feng, F.; Wang, X.; Li, Y. Nanoscale 2019, 11 (16), 7648. doi: 10.1039/c8nr10338k
47. [47]
  Qin, Y.; Chen, K.; Gu, W.; Dong, X.; Lei, R.; Chang, Y.; Bai, X.; Xia, S.; Zeng, L.; Zhang, J.; et al. J. Nanobiotechnol. 2018, 16 (1), 54. doi: 10.1186/s12951-018-0380-z
48. [48]
  Peng, F.; Setyawati, M. I.; Tee, J. K.; Ding, X. G.; Wang, J. P.; Nga, M. E.; Ho, H. K.; Leong, D. T. Nat. Nanotechnol. 2019, 14 (3), 279. doi: 10.1038/s41565-018-0356-z
49. [49]
  Pottler, M.; Fliedner, A.; Schreiber, E.; Janko, C.; Friedrich, R. P.; Bohr, C.; Dollinger, M.; Alexiou, C.; Durr, S. Nanoscale Res. Lett. 2017, 12 (1), 284. doi: 10.1186/s11671-017-2045-5
50. [50]
  Li, Y.; Jing, L.; Yu, Y.; Yu, Y.; Duan, J.; Yang, M.; Geng, W.; Jiang, L.; Li, Q.; Sun, Z. Part. Part. Sys. Charact. 2015, 32 (6), 636. doi: 10.1002/ppsc.201400180
51. [51]
  Xie, H.; Wang, P.; Wu, J. Artif. Cells Nanomed Biotechnol. 2019, 47 (1), 260. doi: 10.1080/21691401.2018.1552594
52. [52]
  Soenen, S. J. H.; Nuytten, N.; De Meyer, S. F.; De Smedt, S. C.; De Cuyper, M. Small 2010, 6 (7), 832. doi: 10.1002/smll.200902084
53. [53]
  Qin, H.; Zhu, C.; An, Z.; Jiang, Y.; Zhao, Y.; Wang, J.; Liu, X.; Hui, B.; Zhang, X.; Wang, Y. Int. J. Nanomed. 2014, 9, 2469. doi: 10.2147/IJN.S59753
54. [54]
  Calzado-Martin, A.; Encinar, M.; Tamayo, J.; Calleja, M.; Paulo, A. S. ACS Nano 2016, 10 (3), 3365. doi: 10.1021/acsnano.5b07162
55. [55]
  Mao, H.; Li, J.; Dulinska-Molak, I.; Kawazoe, N.; Takeda, Y.; Mamiya, H.; Chen, G. Biomater. Sci. 2015, 3 (9), 1284. doi: 10.1039/c5bm00141b
56. [56]
  Ogneva, I. V.; Buravkov, S. V.; Shubenkov, A. N.; Buravkova, L. B. Nanoscale Res. Lett. 2014, 9. doi: 10.1186/1556-276x-9-284
57. [57]
  Tian, X.; Yang, Z.; Duan, G.; Wu, A.; Gu, Z.; Zhang, L.; Chen, C.; Chai, Z.; Ge, C.; Zhou, R. Small 2017, 13 (3). doi: 10.1002/smll.201602133
58. [58]
  Sun, Q.; Kanehira, K.; Taniguchi, A. Sci. Technol. Adv. Mater. 2018, 19 (1), 271. doi: 10.1080/14686996.2018.1444318
59. [59]
  Vieira, L. F. A.; Lins, M. P.; Viana, I.; Dos Santos, J. E.; Smaniotto, S.; Reis, M. Nanoscale Res. Lett. 2017, 12 (1), 200. doi: 10.1186/s11671-017-1982-3

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

纳米粒子与细胞相互作用的力学–化学偶联研究进展

通讯作者: 姚立, yaoli@iccas.ac.cn; 葛茂发, gemaofa@iccas.ac.cn

English

Progress in Chemo–Mechanical Interactions between Nanoparticles and Cells

Corresponding author: Emails: yaoli@iccas.ac.cn(L. Y.); Tel.: +86-10-62588930(L. Y.); Emails: gemaofa@iccas.ac.cn(M. G.)

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

纳米粒子与细胞相互作用的力学–化学偶联研究进展

通讯作者: 姚立, yaoli@iccas.ac.cn; 葛茂发, gemaofa@iccas.ac.cn

English

Progress in Chemo–Mechanical Interactions between Nanoparticles and Cells

Corresponding author: Emails: yaoli@iccas.ac.cn(L. Y.); Tel.: +86-10-62588930(L. Y.); Emails: gemaofa@iccas.ac.cn(M. G.)

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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