引用本文:
Yong CHEN, Yuan Zong LI, Wen Bao CHANG, Yun Xiang CI. STUDIES OF THE INTERACTION BETWEEN METALLOPORPHYRINS OF TMPYP-3 AND SINGLE-STRANDED DNA ON NC MEMBRANE[J]. Chinese Chemical Letters,
1995, 6(2): 123-126.
Citation: Yong CHEN, Yuan Zong LI, Wen Bao CHANG, Yun Xiang CI. STUDIES OF THE INTERACTION BETWEEN METALLOPORPHYRINS OF TMPYP-3 AND SINGLE-STRANDED DNA ON NC MEMBRANE[J]. Chinese Chemical Letters, 1995, 6(2): 123-126.
Citation: Yong CHEN, Yuan Zong LI, Wen Bao CHANG, Yun Xiang CI. STUDIES OF THE INTERACTION BETWEEN METALLOPORPHYRINS OF TMPYP-3 AND SINGLE-STRANDED DNA ON NC MEMBRANE[J]. Chinese Chemical Letters, 1995, 6(2): 123-126.
STUDIES OF THE INTERACTION BETWEEN METALLOPORPHYRINS OF TMPYP-3 AND SINGLE-STRANDED DNA ON NC MEMBRANE
摘要:
We report here a fast staining method for single-stranded DNA fixed on nitrocellulose membrane using M-meso-tetra(3-N-methylpyridyl)-porphyrin(M-TMPyP,M:Mn3+,Fe3+,Pt+).The optimal staining solution consists of 54~108μg/ml M-TMPyP in 25~200 mmol/L phosphate buffer solution(pH 5.0~8.0).When DNA dot on NC membrane is treated with the staining solution at room temperature for 1~2 minutes with continuous stirring,it turns to brown-yellow.10pg per dot of single-stranded DNA is visible.It is a useful tool to study the DNA blotting,and for recognition of the special bands of DNA in a complete electrophoretogram of DNA blot.The staining mechanism is also discussed.
English
STUDIES OF THE INTERACTION BETWEEN METALLOPORPHYRINS OF TMPYP-3 AND SINGLE-STRANDED DNA ON NC MEMBRANE
Abstract:
We report here a fast staining method for single-stranded DNA fixed on nitrocellulose membrane using M-meso-tetra(3-N-methylpyridyl)-porphyrin(M-TMPyP,M:Mn3+,Fe3+,Pt+).The optimal staining solution consists of 54~108μg/ml M-TMPyP in 25~200 mmol/L phosphate buffer solution(pH 5.0~8.0).When DNA dot on NC membrane is treated with the staining solution at room temperature for 1~2 minutes with continuous stirring,it turns to brown-yellow.10pg per dot of single-stranded DNA is visible.It is a useful tool to study the DNA blotting,and for recognition of the special bands of DNA in a complete electrophoretogram of DNA blot.The staining mechanism is also discussed.
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