Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer

Citation: Zhen-bing Li, Yan-hui Xiang, Xian-jing Zhou, Jing-jing Nie, Mao Peng, Bin-yang Du. Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer[J]. Chinese Journal of Polymer Science, 2015, 33(11): 1516-1526. doi: 10.1007/s10118-015-1694-z shu

Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer

1.
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science &
2.
Department of Chemistry, Zhejiang University, Hangzhou 310027, China

通讯作者: Bin-yang Du,

基金项目:
This work was financially supported by the National Natural Science Foundation of China (Nos. 21274129 and 21322406), the Fundamental Research Funds for the Central Universities (No. 2014XZZX003-21), the third level of 2013 Zhejiang Province 151 Talent Project, and Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences.

摘要: The novel microgels, poly[di(ethylene glycol) methyl ether methacrylate-co-2-methoxyethyl acrylate] poly(DEGMMA-co-MEA) microgels, were synthesized. The poly(DEGMMA-co-MEA) microgels were thermo-sensitive and exhibited a volume phase transitive temperature (VPTT) of 14-22 ℃. The incorporation of hydrophobic comonomer MEA shifted the VPTT of poly(DEGMMA-co-MEA) microgels to lower temperatures. The interfacial interaction of poly(DEGMMA-co-MEA) microgels and three model proteins, namely fibrinogen, bovine serum albumin and lysozyme, was investigated by quartz crystal microbalance (QCM). An injection sequence of microgel-after-protein was then established for the real-time study of the interaction of proteins and the microgels at their swollen and collapsed states by using QCM technique. The results indicated that the interfacial interaction of poly(DEGMMA-co-MEA) microgels and adsorbed protein layers was mainly determined by the electrostatic interaction. Because poly(DEGMMA-co-MEA) microgels were negatively charged in Tris-HCl buffer solution (pH = 7.4), the microgels did not adsorb on negatively charged fibrinogen and bovine serum albumin layers but strongly adsorbed on positively charged lysozyme layer. Stronger interaction between lysozyme and the microgels at collapsed state (i.e. at 37 ℃) was observed. Furthermore, the incorporation of MEA might weaken the interaction between poly(DEGMMA-co-MEA) microgels and proteins.

关键词:

English

Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer

1.
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science &
2.
Department of Chemistry, Zhejiang University, Hangzhou 310027, China

Corresponding author: Bin-yang Du,

Received Date: 08 April 2015
Revised Date: 28 April 2015
Available Online: 05 November 2015
Fund Project:
This work was financially supported by the National Natural Science Foundation of China (Nos. 21274129 and 21322406), the Fundamental Research Funds for the Central Universities (No. 2014XZZX003-21), the third level of 2013 Zhejiang Province 151 Talent Project, and Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences.

Abstract: The novel microgels, poly[di(ethylene glycol) methyl ether methacrylate-co-2-methoxyethyl acrylate] poly(DEGMMA-co-MEA) microgels, were synthesized. The poly(DEGMMA-co-MEA) microgels were thermo-sensitive and exhibited a volume phase transitive temperature (VPTT) of 14-22 ℃. The incorporation of hydrophobic comonomer MEA shifted the VPTT of poly(DEGMMA-co-MEA) microgels to lower temperatures. The interfacial interaction of poly(DEGMMA-co-MEA) microgels and three model proteins, namely fibrinogen, bovine serum albumin and lysozyme, was investigated by quartz crystal microbalance (QCM). An injection sequence of microgel-after-protein was then established for the real-time study of the interaction of proteins and the microgels at their swollen and collapsed states by using QCM technique. The results indicated that the interfacial interaction of poly(DEGMMA-co-MEA) microgels and adsorbed protein layers was mainly determined by the electrostatic interaction. Because poly(DEGMMA-co-MEA) microgels were negatively charged in Tris-HCl buffer solution (pH = 7.4), the microgels did not adsorb on negatively charged fibrinogen and bovine serum albumin layers but strongly adsorbed on positively charged lysozyme layer. Stronger interaction between lysozyme and the microgels at collapsed state (i.e. at 37 ℃) was observed. Furthermore, the incorporation of MEA might weaken the interaction between poly(DEGMMA-co-MEA) microgels and proteins.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer

通讯作者: Bin-yang Du,

English

Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer

Corresponding author: Bin-yang Du,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer

通讯作者: Bin-yang Du,

English

Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer

Corresponding author: Bin-yang Du,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

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