引用本文:
杨绍明, 查文玲, 李红, 孙清, 刘斌, 郑龙珍. 半胱氨酸封闭剂为电化学探针的非标记型核酸适配体传感器[J]. 应用化学,
2013, 30(5): 549-554.
doi:
10.3724/SP.J.1095.2013.20299
Citation: YANG Shaoming, ZHA Wenling, LI Hong, SUN Qing, LIU Bin, ZHENG Longzhen. Label-free Aptamer Biosensor Based on Cysteine Blocker as the Electrochemical Redox Probe[J]. Chinese Journal of Applied Chemistry, 2013, 30(5): 549-554. doi: 10.3724/SP.J.1095.2013.20299
Citation: YANG Shaoming, ZHA Wenling, LI Hong, SUN Qing, LIU Bin, ZHENG Longzhen. Label-free Aptamer Biosensor Based on Cysteine Blocker as the Electrochemical Redox Probe[J]. Chinese Journal of Applied Chemistry, 2013, 30(5): 549-554. doi: 10.3724/SP.J.1095.2013.20299
半胱氨酸封闭剂为电化学探针的非标记型核酸适配体传感器
English
Label-free Aptamer Biosensor Based on Cysteine Blocker as the Electrochemical Redox Probe
Abstract:
A novel label-free electrochemical aptamer biosensor was developed by using cysteine blocker as the electrochemical redox probe,and was used for the determination of thrombin. Electrochemical impedance spectroscopy was used to monitor the self-assembly process of aptamer biosensor. The electrochemical behaviors of the biosensor were characterized by the cyclic voltammetry and differential pulse voltammetry. The effects of the incubation time and test pH were investigated on the response of aptamer biosensor. The biosensor shows a good linear range of 10.0~10000 μg/L for thrombin with a detection limit of 2.47 μg/L.
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Key words:
- cysteine
- / blocker
- / redox probe
- / aptamer
- / biosensor
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