Citation: Meng Wu-Yi, Niu Xiu-Tian, Gui Lu-Lu, Lin Zheng-Jiong, Zhu Hong, Zhou Yuan-Cong, Li Gen. Cystallization and Preliminary Diffraction Data of Orthorhombic Crystal Form of Basic Phospholipase A2 from the Venom of Agkistrodon Halys Pallas[J]. Acta Physico-Chimica Sinica, 1996, 12(10): 946-949. doi: 10.3866/PKU.WHXB19961019
English
Cystallization and Preliminary Diffraction Data of Orthorhombic Crystal Form of Basic Phospholipase A2 from the Venom of Agkistrodon Halys Pallas
The basic phospholipase A2 from the venom of Agkistrodon halys pallas possesses the ability to cause hemolysis in contrast to the other two phospholipase A2 from the same venom. A new form of crystals of this enzyme was grown. The crystals belong to space group of P212121 with unit cell parameters of a=9.175nm, b=10.080nm , c=2.287nm.The crystals diffract to high resolution, and are suitable for detailed structural studies. The data were collected up to 0.25nm resolution using synchrotron radiation-imaging plate-Weissenberg camera system. Preliminary analysis reveals the presence of two molecules in the asymmetric uint and the molecule may pack with the smallest dimension approximately parallel to the c axis. The new crystal form is more attractive than the monoclinic one previously reported for crystallographic structure determination as it contains fewer molecules in the asymmetric unit.
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Key words:
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Basic phospholipase A2
- / Agkistrodon halys pallas
- / Hemolysis
- / Crystallization
- / X-ray diffraction
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