微波辅助萃取-超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法快速测定食品接触塑料制品中48种污染物残留

引用本文: 张宪臣, 张朋杰, 时成玉, 华洪波, 容裕棠, 卢俊文, 杨芳. 微波辅助萃取-超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法快速测定食品接触塑料制品中48种污染物残留[J]. 色谱, 2018, 36(7): 634-642. doi: 10.3724/SP.J.1123.2018.01047 shu

Citation: ZHANG Xianchen, ZHANG Pengjie, SHI Chengyu, HUA Hongbo, RONG Yutang, LU Junwen, YANG Fang. Rapid determination of 48 contaminant residues in food contact plastic products by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry coupled with microwave-assisted extraction[J]. Chinese Journal of Chromatography, 2018, 36(7): 634-642. doi: 10.3724/SP.J.1123.2018.01047 shu

微波辅助萃取-超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法快速测定食品接触塑料制品中48种污染物残留

张宪臣 ^1, ,
张朋杰 ^1, ,
时成玉 ^1, ,
华洪波 ^2, ,
容裕棠 ^1, ,
卢俊文 ^1, ,
杨芳 ^1,

1.
中山出入境检验检疫局检验检疫技术中心, 广东中山 528403;
2.
中山市卓雅外语学校, 广东中山 528401
基金项目:
国家质检总局科技计划项目（2015IK052）；中山市社会公益重大项目（2017B1029）；广东检验检疫局科技计划项目（2017GDK24）.

摘要: 利用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法（UPLC-Q Orbitrap HRMS）的同时定性定量功能，建立了食品接触塑料制品中48种污染物残留的检测方法。用甲醇提取目标化合物，经微波辅助萃取，采用CAPCELL PAK MG Ⅱ C18色谱柱（150 mm×2.0 mm，5 μm）分离，以0.04%（v/v）甲酸甲醇溶液和含0.04%（v/v）甲酸与20 mmol/L（v/v）乙酸铵的水溶液为流动相进行梯度洗脱，在加热电喷雾电离（HESI）源、全扫描/实时二级质谱（Full MS/dd-MS²）监测模式下进行检测。结果表明，48种目标化合物在各自范围内线性关系良好，相关系数（r）均≥ 0.9950；检出限为0.1~1.0 μg/kg；平均加标回收率为71.2%~108.8%，相对标准偏差为2.2%~11.8%（n=6）。该法具有操作简单快捷、灵敏度高等优点。

关键词:

English

Rapid determination of 48 contaminant residues in food contact plastic products by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry coupled with microwave-assisted extraction

1.
Inspection and Quarantine Technology Center of Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528403, China;
2.
Zhongshan Zhuoya Foreign Language School, Zhongshan 528401, China
Received Date: 30 January 2018
Fund Project:
Science and Technology Plan Project of General Administration of Quality Supervision Inspection and Quarantine(AQSIQ) of China(No. 2015IK052)

Abstract: An ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q Orbitrap HRMS) method was developed for the rapid determination of 48 contaminant residues in food plastic packaging materials. The samples were extracted with methanol using microwave-assisted extraction. The separation of the 48 contaminants was performed on a CAPCELL PAK MG Ⅱ C18 column (150 mm×2.0 mm, 5 μm) with gradient elution. Methanol (containing 0.04% (v/v) formic acid) and water (containing 0.04% (v/v) formic acid and 20 mmol/L ammonium acetate) were used as mobile phases. The compounds were detected under the full scan/date dependent-MS/MS (Full MS/dd-MS²) mode with a heated electrospray ionization (HESI) source. The calibration curves were linear with correlation coefficients no less than 0.9950. The limits of detection (LODs) ranged from 0.1 to 1.0 μg/kg. The average spiked recoveries of the 48 target compounds were between 71.2% and 108.8%, with relative standard derivations (RSDs) ranging from 2.2% to 11.8% (n=6). Compared to previous methods, this method has advantages of simpler sample preparation and higher sensitivity.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

微波辅助萃取-超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法快速测定食品接触塑料制品中48种污染物残留

English

Rapid determination of 48 contaminant residues in food contact plastic products by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry coupled with microwave-assisted extraction

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微波辅助萃取-超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法快速测定食品接触塑料制品中48种污染物残留

English

Rapid determination of 48 contaminant residues in food contact plastic products by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry coupled with microwave-assisted extraction

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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