磁性反相限进材料的制备及其应用

引用本文: 吴姗姗, 魏缠玲, 赵丽娟, 田央, 王富强, 龚波林. 磁性反相限进材料的制备及其应用[J]. 色谱, 2018, 36(7): 608-614. doi: 10.3724/SP.J.1123.2018.01040 shu

Citation: WU Shanshan, WEI Chanling, ZHAO Lijuan, TIAN Yang, WANG Fuqiang, GONG Bolin. Preparation and application of magnetic reversed-phase restricted access material[J]. Chinese Journal of Chromatography, 2018, 36(7): 608-614. doi: 10.3724/SP.J.1123.2018.01040 shu

磁性反相限进材料的制备及其应用

北方民族大学化学与化学工程学院, 宁夏银川 750021
基金项目:
国家自然科学基金（21565001）.

摘要: 以磁性二氧化硅（Fe₂O₃@SiO₂）为基质，利用表面原子转移自由基聚合技术（SI-ATRP），在改性后的Fe₂O₃@SiO₂内表面接枝甲基丙烯酸十八烷基酯（SMA）、外表面接枝甲基丙烯酸缩甘油酯（GMA），酸解后得到磁性反相限进材料Fe₂O₃@SiO₂-SMA-GMMA，并通过扫描电镜（SEM）、X射线衍射（XRD）、透射电镜（TEM）、振动样品磁强计（VSM）和元素分析对其进行表征。研究表明，Fe₂O₃@SiO₂-SMA-GMMA对牛血清白蛋白（BSA）的排阻能力为90.4%；对磺胺异恶唑（SIZ）、磺胺二甲氧基嘧啶（SDM）、甲氧苄啶（TMP）和磺胺甲基嘧啶（SMR）的最大吸附量分别为2.76、2.24、1.51和1.34 mg/g。Fe₂O₃@SiO₂-SMA-GMMA应用于牛奶和牛血清样品中SIZ、SMR和SDM的分离富集，SIZ、SMR和SDM的加标回收率为88.7%~90.8%，相对标准偏差为3.3%~5.3%。磁性反相限进材料可简化生物基质样品的前处理过程，对血液样品或食品样品等领域的分析检测具有重要价值。

关键词:

English

Preparation and application of magnetic reversed-phase restricted access material

School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan, 750021, China
Received Date: 28 January 2018
Fund Project:
National Natural Science Foundation of China(No. 215605001).

Abstract: Using magnetic silica (Fe₂O₃@SiO₂) as the matrix material, a magnetic reversed-phase restricted access material was prepared via surface-initiation atom transfer radical polymerization (SI-ATRP) on the surface of modified Fe₂O₃@SiO₂. Stearyl methacrylate (SMA) was grafted on the inner surface of the modified Fe₂O₃@SiO₂, and glycidylmethacrylate (GMA) was grafted on the outer surface of the modified Fe₂O₃@SiO₂. The magnetic reversed-phase restricted access material was prepared via acid hydrolysis. This material was characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM) and elemental analysis. The exclusion ability of the opened magnetic reversed-phase restricted access material to bovine serum albumin (BSA) was 90.4%. The maximum adsorbed amounts of sulfisoxazole (SIZ), sulfadimethoxine (SDM), trimethoprim (TMP) and sulfamerazine (SMR) were 2.76, 2.24, 1.51 and 1.34 mg/g, respectively. This material was applied to extract and enrich SIZ, SMR and SDM in milk and bovine serum samples. The spiked recoveries of SIZ, SMR and SDM were 88.7%-90.8%, and the relative standard deviations were 3.3%-5.3%. Thus, the magnetic phase restricted access material can simplify the pretreatment of biological matrix samples, and can be applied to the analysis and detection of blood samples or food samples.

Key words:

solid phase extraction(SPE)
/ magnetic reversed-phase restricted access material
/ surface-initiated atom transfer radical polymerization(SI-ATRP)
/ sulfonamides(SAs)
/ preparation

1. [1] Huang D M, Huang X Y, Gu R R, et al. Chinese Journal of Chromatography, 2014, 32(8):874 黄冬梅, 黄宣运, 顾润润, 等. 色谱, 2014, 32(8):874
2. [2] Lv Y K, Guo Z Y, Wang J Z, et al. Anal Methods, 2015, 7(4):1563
3. [3] Nováková L, Vlcková H. Anal Chim Acta, 2009, 656(1/2):8
4. [4] Zhang X A, Lin W F, Chen S F, et al. Langmuir, 2011, 27(22):13669
5. [5] Cai Y Q, Mou S F. Chinese Journal of Analytical Chemistry, 2005, 33(11):1647 蔡亚岐, 牟世芬. 分析化学, 2005, 33(11):1647
6. [6] Fumes B H, Silva M R, Andrade F N, et al. TrAC-Trends Anal Chem, 2015, 71:9
7. [7] Jia Z P, Zhang Q, Ma J, et al. Chinese Journal of Pharmaceutical Analysis, 2005, 25(12):1476 贾正平, 张强, 马骏, 等. 药物分析杂志, 2005, 25(12):1476
8. [8] Wang C Z, Li M, Wei Y M. University Chemistry, 2014, 29(4):43 王超展, 李溟, 卫引茂. 大学化学, 2014, 29(4):43
9. [9] Callesen A K, Madsen J S, Vach W, et al. Proteomics, 2009, 9(6):1428
10. [10] Gao Q, Feng Y Q. Chinese Journal of Chromatography, 2014, 32(10):1043 高强, 冯钰锜. 色谱, 2014, 32(10):1043
11. [11] Wang C Z, Li M, Xu H H, et al. J Chromatogr A, 2014, 1343(23):195
12. [12] Wang H S, Feng X Y, Wei J P. J Chromatogr A, 2015, 1409(28):132
13. [13] Kanda T, Shirota O, Ohtsu Y, et al. J Chromatogr A, 1996, 722(1/2):115
14. [14] Cass Q B, Lima V V, Oliveira R V, et al. J Chromatogr B, 2003, 798(2):275
15. [15] Zhang X L, Niu H Y, Pan Y Y, et al. Anal Chem, 2010, 82(6):2363
16. [16] Zhang L P, Lv Y K, Wang X H. Journal of Food Safety and Quality, 2014, 5(2):378 张利萍, 吕运开, 王晓虎. 食品安全质量检测学报, 2014, 5(2):378
17. [17] Wang S W, Xu S M, Chen S Z, et al. The Chinese Journal of Nonferrous Metals, 2005, 15(11):1761 王松威, 徐盛明, 陈崧哲, 等. 中国有色金属学报, 2005, 15(11):1761
18. [18] Wei C L, Zhao L J, Qi Y X, et al. Chinese Journal of Chromatography, 2017, 35(2):150 魏缠玲, 赵丽娟, 祁玉霞, 等. 色谱, 2017, 35(2):150
19. [19] Mizutani A, Nagase K, Kikuchi A, et al. J Chromatogr A, 2010, 1217(4):522
20. [20] Cao H, Chen X Z, Zhu Y, et al. New Carbon Materials, 2015, 30(6):572 曹慧, 陈小珍, 朱岩, 等. 新型炭材料, 2015, 30(6):572

扫一扫看文章

计量

PDF下载量: 10
文章访问数: 879
HTML全文浏览量: 115

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

磁性反相限进材料的制备及其应用

English

Preparation and application of magnetic reversed-phase restricted access material

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

磁性反相限进材料的制备及其应用

English

Preparation and application of magnetic reversed-phase restricted access material

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs