在线净化-液相色谱-串联质谱法测定茶叶中5种烟碱类农药残留

引用本文: 李慧思, 黄超群, 蒋沁婷, 钟莺莺, 朱晓雨, 陈笑梅, 莫为民. 在线净化-液相色谱-串联质谱法测定茶叶中5种烟碱类农药残留[J]. 色谱, 2016, 34(3): 263-269. doi: 10.3724/SP.J.1123.2015.10003 shu

Citation: LI Huisi, HUANG Chaoqun, JIANG Qinting, ZHONG Yingying, ZHU Xiaoyu, CHEN Xiaomei, MO Weimin. Determination of five neonicotinoid pesticide residues in tea by online clean-up-liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2016, 34(3): 263-269. doi: 10.3724/SP.J.1123.2015.10003 shu

在线净化-液相色谱-串联质谱法测定茶叶中5种烟碱类农药残留

李慧思 ^1, ,
黄超群 ^{2,
,} ,
蒋沁婷 ^2, ,
钟莺莺 ^3, ,
朱晓雨 ^2, ,
陈笑梅 ^2, ,
莫为民 ^1,

1.
1. 浙江工业大学化学工程学院, 浙江杭州 310014;
2.
2. 浙江出入境检验检疫局, 浙江杭州 310016;
3.
3. 宁波出入境检验检疫局, 浙江宁波 315012

通讯作者: 黄超群

基金项目:
国家质检总局科技计划项目(2015IK302) (2015IK302)

宁波市重大课题(2007C10035). (2007C10035)

摘要: 建立了在线净化-液相色谱-串联质谱测定茶叶中吡虫啉、啶虫脒、噻虫啉、噻虫嗪和噻虫胺5种常见烟碱类农药残留量的方法。样品经水浸泡、乙腈提取和在线净化后,用液相色谱-串联质谱测定。结果表明,本方法对5种烟碱类农药的定量限均为0.01 mg/kg。5种烟碱类农药在1.0~10 μ g/L范围内具有良好的线性关系,相关系数均大于0.998。在0.01、0.02和0.05 mg/kg的添加水平下,回收率为68.0%~113.2%,相对标准偏差为3.2%~7.6%。本方法简便、快速、准确,适用于茶叶样品中烟碱类农药残留量的检测。

关键词:

English

Determination of five neonicotinoid pesticide residues in tea by online clean-up-liquid chromatography-tandem mass spectrometry

1.
1. College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, China;
2.
2. Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou 310016, China;
3.
3. Ningbo Entry-Exit Inspection and Quarantine Bureau, Ningbo 315012, China

Corresponding author: 黄超群

Received Date: 07 October 2015
Fund Project:
国家质检总局科技计划项目(2015IK302)

宁波市重大课题(2007C10035).

Abstract: An online clean-up-liquid chromatography-tandem mass spectrometry method was developed for the determination of five neonicotinoid pesticides (imidacloprid, acetamiprid, thiamethoxam, thiacloprid and clothianidin) in tea. After rehydrated, extracted with acetonitrile, and cleaned-up online, the tea samples were determined by liquid chromatography-tandem mass spectrometry. The results indicated that the limits of quantification for the five neonicotinoid pesticides were 0.01 mg/kg, and the calibration curves were linear in the range of 1.0-10 μ g/L. The spiked recoveries for the five neonicotinoid pesticides from tea were in the range of 68.0%-113.2% with the relative standard deviations between 3.2% and 7.6%. This method is simple, rapid, accurate, and suitable for the simultaneous determination of the five neonicotinoid pesticides in tea.

Key words:

online clean-up
/ liquid chromatography-tandem mass spectrometry (LC-MS/MS)
/ neonicotinoid pesticides
/ tea

1. [1] Liang D S, Jin S H, Duan H X. Chinese Journal of Pesticide Science, 2009, 11(4): 407 梁德胜, 金淑惠, 段红霞. 农药学学报, 2009, 11(4): 407[1] Liang D S, Jin S H, Duan H X. Chinese Journal of Pesticide Science, 2009, 11(4): 407 梁德胜, 金淑惠, 段红霞. 农药学学报, 2009, 11(4): 407
2. [2] Xie W, Qian Y, Ding H Y, et al. Chinese Journal of Analytical Chemistry, 2009, 37(4): 495 谢文, 钱艳, 丁慧瑛, 等. 分析化学, 2009, 37(4): 495[2] Xie W, Qian Y, Ding H Y, et al. Chinese Journal of Analytical Chemistry, 2009, 37(4): 495 谢文, 钱艳, 丁慧瑛, 等. 分析化学, 2009, 37(4): 495
3. [3] Zhang M F, Fan J Y, Zhang H W, et al. World Pesticides, 2009, 31(1): 22 张梅凤, 范金勇, 张宏伟, 等. 世界农药, 2009, 31(1): 22[3] Zhang M F, Fan J Y, Zhang H W, et al. World Pesticides, 2009, 31(1): 22 张梅凤, 范金勇, 张宏伟, 等. 世界农药, 2009, 31(1): 22
4. [4] GB 2763-2014[4] GB 2763-2014
5. [5] European Parliament and Council. Regulation No 396/2005 of the European Parliament and of the Council on Maximum Residue Levels of Pesticides in or on Food and Feed of Plant and Animal Origin and Amending Council Directive 91/414/EEC. (2005-03-16) [2016-01-31]. http://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CONSLEG:2005R0396:20121026[5] European Parliament and Council. Regulation No 396/2005 of the European Parliament and of the Council on Maximum Residue Levels of Pesticides in or on Food and Feed of Plant and Animal Origin and Amending Council Directive 91/414/EEC. (2005-03-16) [2016-01-31]. http://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CONSLEG:2005R0396:20121026
6. [6] European Commission. The Rapid Alert System for Food and Feed. (2005-08-02) [2016-01-31]. http://ec.europa.eu/food/safety/rasff/index_en.htm[6] European Commission. The Rapid Alert System for Food and Feed. (2005-08-02) [2016-01-31]. http://ec.europa.eu/food/safety/rasff/index_en.htm
7. [7] GB/T 23379-2009[7] GB/T 23379-2009
8. [8] NY/T 1724-2009[8] NY/T 1724-2009
9. [9] GB/T 23205-2008[9] GB/T 23205-2008
10. [10] SN/T 2561-2010[10] SN/T 2561-2010
11. [11] Zhang H C, Ai L F, Guo C H, et al. Journal of Instrumental Analysis, 2014, 33(3): 295 张海超, 艾连峰, 郭春海, 等. 分析测试学报, 2014, 33(3): 295[11] Zhang H C, Ai L F, Guo C H, et al. Journal of Instrumental Analysis, 2014, 33(3): 295 张海超, 艾连峰, 郭春海, 等. 分析测试学报, 2014, 33(3): 295
12. [12] Zhang L, Kong X H, He Q, et al. Chinese Journal of Chromatography, 2014, 32(6): 559 张璐, 孔祥虹, 何强, 等. 色谱, 2014, 32(6): 559 [12] Zhang L, Kong X H, He Q, et al. Chinese Journal of Chromatography, 2014, 32(6): 559 张璐, 孔祥虹, 何强, 等. 色谱, 2014, 32(6): 559
13. [13] Duan Y S, Wang B L, Ai L F, et al. Chinese Journal of Chromatography, 2014, 32(6): 647 段永生, 王炳玲, 艾连峰, 等. 色谱, 2014, 32(6): 647 [13] Duan Y S, Wang B L, Ai L F, et al. Chinese Journal of Chromatography, 2014, 32(6): 647 段永生, 王炳玲, 艾连峰, 等. 色谱, 2014, 32(6): 647
14. [14] Ai L F, Ma Y S, Chen R C, et al. Chinese Journal of Analytical Chemistry, 2013, 41(8): 1194 艾连峰, 马育松, 陈瑞春, 等. 分析化学, 2013, 41(8): 1194[14] Ai L F, Ma Y S, Chen R C, et al. Chinese Journal of Analytical Chemistry, 2013, 41(8): 1194 艾连峰, 马育松, 陈瑞春, 等. 分析化学, 2013, 41(8): 1194
15. [15] Roach J A, Dibussolo J M, Krynitsky A, et al. J Chromatogr A, 2011, 1218: 4284 [15] Roach J A, Dibussolo J M, Krynitsky A, et al. J Chromatogr A, 2011, 1218: 4284
16. [16] Bousova K, Senyuva H, Mittendorf K. J Chromatogr A, 2013, 1274: 19 [16] Bousova K, Senyuva H, Mittendorf K. J Chromatogr A, 2013, 1274: 19

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沈阳化工大学材料科学与工程学院沈阳 110142

在线净化-液相色谱-串联质谱法测定茶叶中5种烟碱类农药残留

通讯作者: 黄超群

English

Determination of five neonicotinoid pesticide residues in tea by online clean-up-liquid chromatography-tandem mass spectrometry

Corresponding author: 黄超群

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

在线净化-液相色谱-串联质谱法测定茶叶中5种烟碱类农药残留

通讯作者: 黄超群

English

Determination of five neonicotinoid pesticide residues in tea by online clean-up-liquid chromatography-tandem mass spectrometry

Corresponding author: 黄超群

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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