高效液相色谱法测定化妆品中17种紫外线吸收剂

引用本文: 毛希琴, 边海涛, 曲宝成. 高效液相色谱法测定化妆品中17种紫外线吸收剂[J]. 色谱, 2013, 31(8): 775-780. doi: 10.3724/SP.J.1123.2013.02011 shu

Citation: MAO Xiqin, BIAN Haitao, QU Baocheng. Determination of 17 ultraviolet-filters in cosmetics by high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2013, 31(8): 775-780. doi: 10.3724/SP.J.1123.2013.02011 shu

高效液相色谱法测定化妆品中17种紫外线吸收剂

通讯作者: 毛希琴

基金项目:
国家食品药品监督管理局专项资金项目.

摘要: 为避免使用四氢呋喃和高氯酸这样高腐蚀性的溶剂作流动相,本文从样品前处理方法、色谱柱的选择和流动相条件的优化等方面对《化妆品卫生规范》规定的15种紫外线吸收剂的高效液相色谱测定方法进行了改进,并将目标物检测范围扩大为17种。样品用四氢呋喃/甲醇/水体系提取,离心过滤后,以Phenomenex PFP柱(250 mm×4.6 mm, 5 μm)为分离色谱柱,以20%异丙醇的甲醇溶液(含0.1%甲酸)和水溶液(含0.1%甲酸)作为流动相,梯度洗脱,以311 nm和280 nm为检测波长,进行定性和定量分析。回收率试验及实际阳性样品测定均表明将本方法用于化妆品中17种紫外线吸收剂的测定,结果准确、可靠。与《化妆品卫生规范》方法相比,普通液相色谱仪无需改进即可实现17种紫外线吸收剂的同时检测,增加了方法的普适性,同时所有目标化合物全部基线分离,分离度的提高增加了定量的准确性。

关键词:

English

Determination of 17 ultraviolet-filters in cosmetics by high performance liquid chromatography

1.
Dalian Institute of Product Quality Supervision & Inspection, Dalian 116023, China

Corresponding author: 毛希琴

Received Date: 06 February 2013
Fund Project:
国家食品药品监督管理局专项资金项目.

Abstract: An optimum HPLC method was developed for the simultaneous determination of 17 ultraviolet (UV)-filters in cosmetics, in which two UV-filters were included more than the 15 UV-filters specified in the Hygenic Standard for Cosmetics (HSC). Our research focused on the optimization of sample processing, chromatographic column, and mobile phase, in order to avoid the use of highly corrosive solvents as tetrahydrofuran (THF) and perchloric acid, which are used in the HSC method. The cosmetic sample was extracted by the mixed solution of THF/methanol/water. The target chemicals were separated on a Phenomenex PFP column (250 mm×4.6 mm, 5 μm) in gradient elution mode using water solution (containing 0.1% formic acid) and 20% methanol solution of isopropyl alcohol (containing 0.1% formic acid) as mobile phases. The detection wavelengths were set at 311 nm and 280 nm. The results of recovery tests and samples analysis indicated that the developed method can be used to simultaneously determine the 17 UV-filters in cosmetics, accurately and reliably. It is a more universal analytical method than the HSC method because common HPLC pump without improvement to adapt to corrosive THF, can be used. At the same time, the quantitative accuracy can be improved because baseline separation of all target chemicals was achieved.

Key words:

high performance liquid chromatography (HPLC)
/ ultraviolet-filters
/ cosmetics

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

高效液相色谱法测定化妆品中17种紫外线吸收剂

通讯作者: 毛希琴

English

Determination of 17 ultraviolet-filters in cosmetics by high performance liquid chromatography

Corresponding author: 毛希琴

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

高效液相色谱法测定化妆品中17种紫外线吸收剂

通讯作者: 毛希琴

English

Determination of 17 ultraviolet-filters in cosmetics by high performance liquid chromatography

Corresponding author: 毛希琴

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

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