高效液相色谱-蒸发光散射法测定食品中的单糖、双糖、低聚果糖和糖醇

引用本文: 丁洪流, 李灿, 金萍, 袁丽红, 姚永青, 陈英, 李培. 高效液相色谱-蒸发光散射法测定食品中的单糖、双糖、低聚果糖和糖醇[J]. 色谱, 2013, 31(8): 804-808. doi: 10.3724/SP.J.1123.2013.01013 shu

Citation: DING Hongliu, LI Can, JIN Ping, YUAN Lihong, YAO Yongqing, CHEN Ying, LI Pei. Simultaneous determination of monosaccharides, disaccharides, oligosaccharides and sugar alcohols in foods by high performance liquid chromatography with evaporative light-scattering detection[J]. Chinese Journal of Chromatography, 2013, 31(8): 804-808. doi: 10.3724/SP.J.1123.2013.01013 shu

高效液相色谱-蒸发光散射法测定食品中的单糖、双糖、低聚果糖和糖醇

丁洪流 ^{1,
,} ,
李灿 ^2, ,
金萍 ^1, ,
袁丽红 ^2, ,
姚永青 ^1, ,
陈英 ^1, ,
李培 ^1,

通讯作者: 丁洪流

基金项目:
国家质检总局科技计划项目(2010QK179). (2010QK179)

摘要: 建立了食品中常用的木糖、果糖、葡萄糖、蔗糖、麦芽糖、乳糖、蔗果三糖、蔗果四糖、蔗果五糖、赤藓糖醇、木糖醇、甘露糖醇、麦芽糖醇等13种单糖、双糖、低聚果糖和糖醇的高效液相色谱同时分离检测的方法。该法采用NH₂色谱柱,以乙腈-水为流动相梯度洗脱,蒸发光散射检测器检测;13种糖在0.1~5 g/L内均具有良好的线性关系,检出限均在0.1 g/L以下,精密度(RSD)为2.69%~7.21%,回收率为96.1%~105.2%,结果较为理想。将该法用于实际样品检测,结果显示食品标签明示和实际成分相差较大。

关键词:

English

Simultaneous determination of monosaccharides, disaccharides, oligosaccharides and sugar alcohols in foods by high performance liquid chromatography with evaporative light-scattering detection

DING Hongliu ^{1,
,} ,
LI Can ^2, ,
JIN Ping ^1, ,
YUAN Lihong ^2, ,
YAO Yongqing ^1, ,
CHEN Ying ^1, ,
LI Pei ^1,

1.
1. Suzhou Institute of Supervision and Inspection on Product Quality, Suzhou 215000, China;

Corresponding author: 丁洪流

Received Date: 07 January 2013
Fund Project:
国家质检总局科技计划项目(2010QK179).

Abstract: A simple and efficient method was established based on high performance liquid chromatography (HPLC) to separate and detect thirteen analytes of monosaccharides, disaccharides, oligosaccharides and sugar alcohols (xylose, fructose, glucose, sucrose, maltose, lactose, 1-kestose, nystose, 1F-fructofuranosyl nystose, erythritol, mannitol, xylitol, maltitol) in foods. The separation was performed on an NH₂ column with the gradient elution of acetonitrile-water as the mobile phases. The analytes were detected by an evaporative light-scattering detector (ELSD). All the thirteen sugars had good linearities within 0.1-5 g/L with the correlation coefficients between 0.9901-0.9996. The limits of detection (LOD) were all less than 0.1 g/L. The precisions of the method expressed as RSDs were in the range of 2.69%-7.21%. The recoveries of the thirteen analytes spiked in real samples ranged from 96.1% to 105.2%. This method was applied to the actual sample testings and the results showed the food labels were greatly different from the actual compositions.

Key words:

high performance liquid chromatography (HPLC)
/ evaporative light-scattering detection (ELSD)
/ sugar
/ sugar alcohol
/ food

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沈阳化工大学材料科学与工程学院沈阳 110142

高效液相色谱-蒸发光散射法测定食品中的单糖、双糖、低聚果糖和糖醇

通讯作者: 丁洪流

English

Simultaneous determination of monosaccharides, disaccharides, oligosaccharides and sugar alcohols in foods by high performance liquid chromatography with evaporative light-scattering detection

Corresponding author: 丁洪流

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

高效液相色谱-蒸发光散射法测定食品中的单糖、双糖、低聚果糖和糖醇

通讯作者: 丁洪流

English

Simultaneous determination of monosaccharides, disaccharides, oligosaccharides and sugar alcohols in foods by high performance liquid chromatography with evaporative light-scattering detection

Corresponding author: 丁洪流

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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