引用本文:
文雯, 高晓亚, 张爱平, 宋志英, 孙彦平. 纳米TiO2光催化氧化对胃癌细胞周期的影响[J]. 应用化学,
2012, 29(6): 663-667.
doi:
10.3724/SP.J.1095.2012.00324
Citation: WEN Wen, GAO Xiaoya, ZHANG Aiping, SONG Zhiying, SUN Yanping. Effects of Nano-TiO2 Photocatalytic Oxidization on the Cell Cycle of BGC-823 Gastric Cancer Cells[J]. Chinese Journal of Applied Chemistry, 2012, 29(6): 663-667. doi: 10.3724/SP.J.1095.2012.00324
Citation: WEN Wen, GAO Xiaoya, ZHANG Aiping, SONG Zhiying, SUN Yanping. Effects of Nano-TiO2 Photocatalytic Oxidization on the Cell Cycle of BGC-823 Gastric Cancer Cells[J]. Chinese Journal of Applied Chemistry, 2012, 29(6): 663-667. doi: 10.3724/SP.J.1095.2012.00324
纳米TiO2光催化氧化对胃癌细胞周期的影响
摘要:
以胃癌细胞系BGC-823为研究对象,采用倒置相差显微镜观察纳米TiO2光催化氧化前后BGC-823细胞形态的变化,通过流式细胞仪考察其对BGC-823细胞凋亡率及细胞周期的影响。结果表明,纳米TiO2光催化氧化使BGC-823细胞呈受激坏死或凋亡晚期继发性坏死状态;导致BGC-823细胞周期阻滞于G2/M期并诱导细胞凋亡,从而抑制其DNA合成及增殖分化进程。当TiO2比负载量为9.8×10-7g/cm2、光照时间为40 min时,G2/M期阻滞较为显著,细胞凋亡率达24.34%。
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关键词:
- 光催化氧化
- / 纳米TiO2
- / 胃癌细胞BGC-823
- / 细胞周期
- / 细胞凋亡
English
Effects of Nano-TiO2 Photocatalytic Oxidization on the Cell Cycle of BGC-823 Gastric Cancer Cells
Abstract:
In order to explore the mechanisms of killing cancer cells via nano-TiO2 photocatalytic oxidization,gastric cancer cell line BGC-823 was cultured as research object in this study.The morphologic changes were observed by inverted phase-contrast microscope,the apoptotic rates and cell cycle of BGC-823 cells were examined by flow cytometer.The results showed that nano-TiO2 photocatalytic oxidation caused the necrosis or secondary necrosis of BGC-823 cells,and inhibited the synthesis of DNA and their proliferation process,through arresting the G2/M phase and inducing apoptosis of BGC-823 cells.Significant arrest was observed and the apoptotic rates of BGC-823 cells reached 24.34% when the coated amount of TiO2 was 9.8×10-7 g/cm2 and the irradiation time was 40 min.
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Key words:
- photocatalytic oxidization
- / nano-TiO2
- / gastric cancer cell line BGC-823
- / cell cycle
- / apoptosis
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