Abstract: The complexation of functional groups of polymers to metal ions was exploited to in situ prepare metal particles on the electrode surface. Poly(anthranilic acid)(PoABA) was first electrodeposited onto a glassy carbon electrode(GCE). Then copper ions(Cu²⁺) were chemically adsorbed within the PoABA film, and subsequently reduced by hydrazine hydrate forming copper particles(Cu⁰). The surface morphology and element composition of PoABA-Cu⁰ composite film was characterized by scanning electron microscopy and energy-dispersive X-ray ananlysis. The electrochemical properties of the PoABA-Cu⁰ modified electrode and its application for the detection of H₂O₂ were studied. The results show that there are well-distributed Cu particles in the PoABA film; the modified electrode exhibits well performance toward H₂O₂ electrocatalysis. When the number of potential scans for the polymerization of o-aminobenzoic acid is 10, the adsorption time of Cu²⁺ is 10 min, and the working voltage is-0.3 V, the PoABA-Cu⁰ modified electrode displays the best electroananlytic performance toward H₂O₂, where the linear H₂O₂ concentration range is 5.0×10^-5~1.0×10^-2 mol/L, the sensitivity is 96.34 μA·L/(mmol·cm), and the detection limit is 5.0×10^-5 mol/L. Moreover, the sensing electrode shows a relatively good stability.