基于Au@Pt纳米粒子-双亲性气凝胶的模拟酶可视化检测抗坏血酸

引用本文: 周晨雨, 房琦, 张玉, 杜衍. 基于Au@Pt纳米粒子-双亲性气凝胶的模拟酶可视化检测抗坏血酸[J]. 分析化学, 2021, 49(6): 982-991. doi: 10.19756/j.issn.0253-3820.211155 shu

Citation: ZHOU Chen-Yu, FANG Qi, ZHANG Yu, DU Yan. Visualized Detection of Ascorbic Acid by Oxidase Mimics Based on Au@Pt Nanoparticles and Amphiphilic Aerogel[J]. Chinese Journal of Analytical Chemistry, 2021, 49(6): 982-991. doi: 10.19756/j.issn.0253-3820.211155 shu

基于Au@Pt纳米粒子-双亲性气凝胶的模拟酶可视化检测抗坏血酸

周晨雨 ^1,2, ,
房琦 ^1,2, ,
张玉 ^{1,2,
,} ,
杜衍 ^{1,2,
,}

1.
中国科学院长春应用化学研究所, 电分析化学国家重点实验室, 长春 130022;
2.
中国科学技术大学应用化学与工程学院, 合肥 230026

通讯作者: 张玉,E-mail:zhangyu_jcb@ciac.ac.cn; 杜衍,E-mail:duyan@ciac.ac.cn

基金项目:
国家自然科学基金项目（Nos.21874129，31960506）和吉林省科技厅国际科技合作项目（No.20200801044GH）资助。

摘要: 采用水热法在双亲性聚乙烯醇气凝胶（PAA）载体上原位生长Au@Pt纳米粒子，制备Au@Pt-PAAC复合纳米材料。PAA载体上丰富的含氧基团为纳米粒子的生长提供了成核位点，而核壳结构的Au@Pt纳米粒子，一方面由于协同效应提高了材料的类氧化酶活性，另一方面减少了铂的用量，降低了催化剂成本。基于Au@Pt-PAAC材料的类氧化酶活性构筑了比色传感器用于还原性物质抗坏血酸（AA）的可视化检测。在优化的实验条件下，检测AA的线性范围为10~100 μmol/L，检出限为8.77 μmol/L。将本方法用于饮料和维生素C片中的AA含量检测，所得实验结果与样品标示含量一致，因此可用于总抗氧化能力（TAC）的评价。本方法简单、直观、抗干扰能力强，具有良好的实用性。

关键词:

English

Visualized Detection of Ascorbic Acid by Oxidase Mimics Based on Au@Pt Nanoparticles and Amphiphilic Aerogel

ZHOU Chen-Yu ^1,2, ,
FANG Qi ^1,2, ,
ZHANG Yu ^{1,2,
,} ,
DU Yan ^{1,2,
,}

1.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2.
School of Applied Chemistry and Engineering, University of Science & Technology of China, Hefei 230026, China

Corresponding author: ZHANG Yu, ; DU Yan,

Received Date: 01 March 2021
Revised Date: 25 March 2021
Fund Project:
Supported by the National Natural Science Foundation of China(Nos.21874129, 31960506) and the Department of Science and Technology of Jilin Province, China (No.20200801044GH).

Abstract: Au@Pt nanoparticles were grown in-situ on an amphiphilic polyvinyl alcohol aerogel (PAA) surface by a hydrothermal method to prepare an oxidase mimic (Au@Pt-PAAC) and was further applied to the visual detection of ascorbic acid. The abundant oxygen-containing functional groups on the PAA carrier provided nucleation sites for the growth of nanoparticles. Also, the core-shell structure of Au@Pt nanoparticles improved the oxidase-like activity of the material due to the synergistic effect and reduced the dosage of platinum, thus helping with cost control. Based on the oxidase-like activity of Au@Pt-PAAC material, a colorimetric sensor for the visual detection of ascorbic acid was constructed. Under the optimized experimental conditions, the linear range for the detection of ascorbic acid was 10-100 μmol/L, and the detection limit was 8.77 μmol/L. The sensor was also employed to evaluate the total antioxidant capacity of beverages and vitamin C tablets, with the experiments showing consistent results with the content of the ingredients in the sample instructions. The method established here is simple and visualized, with good anti-interference ability, and can be used to efficiently detect antioxidants.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于Au@Pt纳米粒子-双亲性气凝胶的模拟酶可视化检测抗坏血酸

通讯作者: 张玉,E-mail:zhangyu_jcb@ciac.ac.cn; 杜衍,E-mail:duyan@ciac.ac.cn

English

Visualized Detection of Ascorbic Acid by Oxidase Mimics Based on Au@Pt Nanoparticles and Amphiphilic Aerogel

Corresponding author: ZHANG Yu, ; DU Yan,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于Au@Pt纳米粒子-双亲性气凝胶的模拟酶可视化检测抗坏血酸

通讯作者: 张玉,E-mail:zhangyu_jcb@ciac.ac.cn; 杜衍,E-mail:duyan@ciac.ac.cn

English

Visualized Detection of Ascorbic Acid by Oxidase Mimics Based on Au@Pt Nanoparticles and Amphiphilic Aerogel

Corresponding author: ZHANG Yu, ; DU Yan,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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