基于表面印迹聚合物的电化学传感器用于乙型副伤寒沙门氏菌的检测

引用本文: 刘婷, 林晓惠, 陈谦, 卞晓军, 颜娟. 基于表面印迹聚合物的电化学传感器用于乙型副伤寒沙门氏菌的检测[J]. 分析化学, 2022, 50(1): 127-135. doi: 10.19756/j.issn.0253-3820.210715 shu

Citation: LIU Ting, LIN Xiao-Hui, CHEN Qian, BIAN Xiao-Jun, YAN Juan. An Electrochemical Sensor Based on Surface Molecularly Imprinted Polymer for Detection of Salmonella Paratyphi B[J]. Chinese Journal of Analytical Chemistry, 2022, 50(1): 127-135. doi: 10.19756/j.issn.0253-3820.210715 shu

基于表面印迹聚合物的电化学传感器用于乙型副伤寒沙门氏菌的检测

刘婷 ^1, ,
林晓惠 ^1, ,
陈谦 ^1, ,
卞晓军 ^{1,2,3,
,} ,
颜娟 ^{1,2,3,
,}

1.
上海海洋大学食品学院, 上海 201306;
2.
上海水产品加工及贮藏工程技术研究中心, 上海 201306;
3.
农业部水产品贮藏保鲜质量安全风险评估实验室(上海), 上海 201306

通讯作者: 卞晓军,E-mail:j-yan@shou.edu.cn; 颜娟,E-mail:xjbian@shou.edu.cn

基金项目:
国家自然科学基金项目（No.21775102）和上海市自然科学基金项目（No.20ZR1424100）资助。

摘要: 致病菌感染引发的食源性疾病已成为全球性的食品安全和公共卫生问题，因此，构建快速、灵敏的致病菌分析方法具有重要意义。本研究结合分子印迹技术与电化学阻抗技术，以噻吩-3-乙醇（TE）为单体，乙型副伤寒沙门氏菌（S.Paratyphi B）为模板，采用一步电聚合法在玻碳电极表面快速、便捷地制备了一层具有特异性识别能力的细菌印迹膜，据此构建了一种快速、灵敏、无标记检测沙门氏菌的新方法。采用电化学阻抗和扫描电子显微镜对细菌印迹膜的形成过程进行了表征。在最佳实验条件下，细菌印迹传感器具有识别快速（仅10 min）、线性范围宽（1.0×10¹~1.0×10⁶ CFU/mL）、检出限低（约2.0 CFU/mL）、选择性好等良好的分析性能，用于实际样品（饮用水和脱脂奶粉）中沙门氏菌的检测效果良好，回收率高（93.0%~114.9%）。与现有的细菌生物传感方法相比，此传感器具有制备高效（印迹膜的形成仅需15 min）、成本低、环境友好等优点，在细菌检测方面具有较大的应用潜力。

关键词:

English

An Electrochemical Sensor Based on Surface Molecularly Imprinted Polymer for Detection of Salmonella Paratyphi B

1.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
2.
Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China;
3.
Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China

Corresponding author: BIAN Xiao-Jun, ; YAN Juan,

Received Date: 30 August 2021
Revised Date: 24 October 2021
Fund Project:
Supported by the National Natural Science Foundation of China (No.21775102) and the Natural Science Foundation of Shanghai Municipal, China (No.20ZR1424100).

Abstract: Salmonella Paratyphi B (S.Paratyphi B) is one of the most widespread pathogenic bacteria causing foodborne disease. It is of great significance to construct a rapid and sensitive analysis method for detection of S.Paratyphi B. In this work, a simple, efficient and cost-effective surface bacteria-imprinted film-based impedance sensor was prepared. First of all, with S.Paratyphi B as the template and thiophene-3-ethanol as the functional monomer, the polythiophene and bacteria were co-deposited on the surface of glassy carbon electrode by cyclic voltammetry. After in situ removal of the template bacteria, a surface bacterial imprinting film was formed on the electrode surface, which could specifically recognize the target bacteria and successfully realize the label-free detection of the target bacteria. Under the optimal experimental conditions, this bacterial imprinted impedance sensor showed good analysis performance, e.g., fast recognition (10 min), wide linear range (1.0×10¹-1.0×10⁶ CFU/mL), low detection limit (approximately 2.0 CFU/mL), and good selectivity. In addition, this impedance sensor could be successfully applied to detection of S.Paratyphi B in real samples with good recovery. Compared with the existing bacterial biosensor, the impedance sensor here had many advantages such as high preparation efficiency (only 5 min for imprinted film formation), low cost and environmental friendliness, showing potential applications in detection of foodborne pathogenic bacteria.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于表面印迹聚合物的电化学传感器用于乙型副伤寒沙门氏菌的检测

通讯作者: 卞晓军,E-mail:j-yan@shou.edu.cn; 颜娟,E-mail:xjbian@shou.edu.cn

English

An Electrochemical Sensor Based on Surface Molecularly Imprinted Polymer for Detection of Salmonella Paratyphi B

Corresponding author: BIAN Xiao-Jun, ; YAN Juan,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于表面印迹聚合物的电化学传感器用于乙型副伤寒沙门氏菌的检测

通讯作者: 卞晓军,E-mail:j-yan@shou.edu.cn; 颜娟,E-mail:xjbian@shou.edu.cn

English

An Electrochemical Sensor Based on Surface Molecularly Imprinted Polymer for Detection of Salmonella Paratyphi B

Corresponding author: BIAN Xiao-Jun, ; YAN Juan,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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