在线测定秦皮甲素和秦皮乙素水解反应速率常数的扫集-流动注射-芯片毛细管胶束电动色谱方法

引用本文: 朱华东, 陈永雷, 陈宏丽, 陈兴国. 在线测定秦皮甲素和秦皮乙素水解反应速率常数的扫集-流动注射-芯片毛细管胶束电动色谱方法[J]. 分析化学, 2016, 44(4): 597-603. doi: 10.11895/j.issn.0253-3820.160142 shu

Citation: ZHU Hua-Dong, CHEN Yong-Lei, CHEN Hong-Li, CHEN Xing-Guo. On-line Study of Aesculin and Aesculetin Hydrolysis Using Sweeping-Flow Injection-chip Micellar Electrokinetic Chromatography[J]. Chinese Journal of Analytical Chemistry, 2016, 44(4): 597-603. doi: 10.11895/j.issn.0253-3820.160142 shu

在线测定秦皮甲素和秦皮乙素水解反应速率常数的扫集-流动注射-芯片毛细管胶束电动色谱方法

1.
兰州大学化学化工学院, 兰州 730000

通讯作者: 陈兴国

基金项目:
本文系国家自然科学基金(No.21375053)资助 (No.21375053)

摘要: 建立了在线测定秦皮甲素和秦皮乙素水解反应速率常数的扫集-流动注射-胶束电动色谱新方法。该方法进样频率为12次/h,可在5 min内完全分离反应体系中的所有组分,30 min即可完成一个温度下的反应速率常数测定。由于该在线测定方法不需要终止水解反应,通过一次连续进样分析得到水解反应过程电泳谱图,从而可以获得水解反应过程中的一些信息。在最佳条件时,用0.1 mol/L KOH为催化剂,测得25,30,35,40和45℃时秦皮甲素的水解反应速率常数分别为3.65×10^-2/min、5.24×10^-2/min、7.12×10^-2/min、10.5×10^-2/min和16.3×10^-2/min,活化能为58.57 kJ/mol。用10 mmol/L KOH为催化剂,测得15,20,25,30和35℃时秦皮乙素的水解反应速率常数分别为2.26×10^-2/min,2.85×10^-2/min,3.55×10^-2/min,4.38×10^-2/min和5.29×10^-2/min,活化能为31.55 kJ/mol。

关键词:

English

On-line Study of Aesculin and Aesculetin Hydrolysis Using Sweeping-Flow Injection-chip Micellar Electrokinetic Chromatography

1.
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China

Corresponding author: 陈兴国

Received Date: 28 February 2016
Fund Project:
本文系国家自然科学基金(No.21375053)资助

Abstract: Aesculin and aesculetin hydrolysis were studied on-line using sweeping-flow injection-chip micellar electrokinetic chromatography(Sweeping-FI-chip MEKC). With this method, all the analytes in the sample are well separated within 5 min, and a sampling rate of 12 per hour is achieved. At this sampling frequency, a rate constant can be obtained within 30 min. In contrast to off-line methods, it was quite time-saving. And the aesculin hydrolysis mixture solution is directly injected and analyzed without quenching the reaction, thus the information of the hydrolysis process can be obtained from the electrophorogram of a sequence injection. Under the optimum conditions, the hydrolysis rate constants for aesculin at 25, 30, 35, 40 and 45℃ using 0.1 mol/L KOH as hydrolyte are obtained as 3.65×10^-2/min, 5.24×10^-2/min, 7.12×10^-2/min, 10.5×10^-2/min and 16.3×10^-2/min, respectively. The activation energy for aesculin hydrolysis is 58.57 kJ/mol. The hydrolysis rate constants for aesculetin at 15, 20, 25, 30 and 35℃ using 10 mmol/L KOH as hydrolyte are obtained as 2.26×10^-2/min, 2.85×10^-2/min, 3.55×10^-2/min, 4.38×10^-2/min and 5.29×10^-2/min, respectively. The activation energy for aesculin hydrolysis is 31.55 kJ/mol.

Key words:

Sweeping-flow injection-chip micellar electrokinetic chromatography
/ On-line hydrolysis
/ Aesculin
/ Aesculetin
/ Rate constant

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沈阳化工大学材料科学与工程学院沈阳 110142

在线测定秦皮甲素和秦皮乙素水解反应速率常数的扫集-流动注射-芯片毛细管胶束电动色谱方法

通讯作者: 陈兴国

English

On-line Study of Aesculin and Aesculetin Hydrolysis Using Sweeping-Flow Injection-chip Micellar Electrokinetic Chromatography

Corresponding author: 陈兴国

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

在线测定秦皮甲素和秦皮乙素水解反应速率常数的扫集-流动注射-芯片毛细管胶束电动色谱方法

通讯作者: 陈兴国

English

On-line Study of Aesculin and Aesculetin Hydrolysis Using Sweeping-Flow Injection-chip Micellar Electrokinetic Chromatography

Corresponding author: 陈兴国

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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