基于微流控芯片的磁性没食子酸丙酯分子印迹聚合物的制备及应用

引用本文: 江丹丹, 宋乃忠, 王皓, 贾琼. 基于微流控芯片的磁性没食子酸丙酯分子印迹聚合物的制备及应用[J]. 分析化学, 2016, 44(4): 647-653. doi: 10.11895/j.issn.0253-3820.160081 shu

Citation: JIANG Dan-Dan, SONG Nai-Zhong, WANG Hao, JIA Qiong. Preparation and Application of Magnetic Propyl Gallate Molecularly Imprinted Polymers in Microchip[J]. Chinese Journal of Analytical Chemistry, 2016, 44(4): 647-653. doi: 10.11895/j.issn.0253-3820.160081 shu

基于微流控芯片的磁性没食子酸丙酯分子印迹聚合物的制备及应用

江丹丹 ^1, ,
宋乃忠 ^1, ,
王皓 ^2, ,
贾琼 ^{1,
,}

1.
¹ 吉林大学化学学院, 长春 130012;
2.
² 吉林大学生命科学学院, 长春 130012

通讯作者: 贾琼

基金项目:
本文系国家自然科学基金(No.21575049) (No.21575049)

吉林省科技发展计划项目(No.20140101112JC)资助 (No.20140101112JC)

摘要: 以3-(甲基丙烯酸酰氧)丙基三甲氧基硅烷修饰的Fe₃O₄为载体,没食子酸丙酯为模板分子,采用表面印迹技术制备了核壳结构的磁性没食子酸丙酯分子印迹聚合物(Fe₃O₄@SiO₂-MIPS),并将该磁性分子印迹聚合物作为吸附剂引入到微流控芯片中,与HPLC联用,实现对痕量酚类抗氧化剂的分离富集。采用扫描电镜、磁滞回线、热重分析、红外光谱及X射线衍射对该磁性分子印迹聚合物表征。在最佳萃取条件下(样品体积3 mL,样品溶液pH=5.0,样品流速为0.5 mL/min,洗脱液流速为0.3 mL/min),该聚合物对酚类抗氧化剂具有较强的富集作用。3种酚类抗氧化剂在10-5000 ng/mL范围内呈现良好的线性关系,线性相关系数均大于0.9901,日内和日间相对标准偏差(n=5)分别低于2.8%和3.5%。将本方法用于化妆水中酚类抗氧化剂的分析检测,加标回收率在87.9%-104.0%之间,相对标准偏差(n=3)小于3.7%。

关键词:

English

Preparation and Application of Magnetic Propyl Gallate Molecularly Imprinted Polymers in Microchip

1.
¹ College of Chemistry, Jilin University, Changchun 130012, China;
2.
² College of Life Sciences, Jilin University, Changchun 130012, China

Corresponding author: 贾琼

Received Date: 31 January 2016
Fund Project:
本文系国家自然科学基金(No.21575049)

吉林省科技发展计划项目(No.20140101112JC)资助

Abstract: 3-Methacryloxypropyltrimethoxy silane modified Fe₃O₄ was employed as the carrier material to prepare core-shell magnetic molecularly imprinted polymers(Fe₃O₄@SiO₂-MIPS) by using a surface molecular imprinting technology with propyl gallate as the template. The magnetic composites were loaded into a microchip and coupled with high-performance liquid chromatography to separate and preconcentrate synthetic phenolic antioxidants(SPAs). The structures of the resulting complex were characterized by scanning electron microscopy, vibrating sample magnetoeter, thermogravimetric analysis/differential thermal gravity analysis, Fourier-transformed infrared spectroscopy and X-ray diffraction spectrometry. Under the optimal conditions(3 mL of sample solution, sample pH of 5.0, sample flow rate of 0.5 mL/min, and eluent flow rate of 0.3 mL/min), the magnetic MIPS displayed enhanced adsorption ability for phenolic antioxidants. The calibration curves showed good linearity with correlation coefficients greater than 0.9901. Intra-day and inter-day relative standard deviations(RSD) were less than 2.8% and 3.5%, respectively. The present method was applied to the determination of phenolic antioxidants in cosmetics samples, illustrating satisfactory recovery(87.9%-104.0%) and reproducibility(RSD less than 3.7%).

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于微流控芯片的磁性没食子酸丙酯分子印迹聚合物的制备及应用

通讯作者: 贾琼

English

Preparation and Application of Magnetic Propyl Gallate Molecularly Imprinted Polymers in Microchip

Corresponding author: 贾琼

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于微流控芯片的磁性没食子酸丙酯分子印迹聚合物的制备及应用

通讯作者: 贾琼

English

Preparation and Application of Magnetic Propyl Gallate Molecularly Imprinted Polymers in Microchip

Corresponding author: 贾琼

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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