南极磷虾油中总砷含量及砷形态分析

引用本文: 王松, LI Ke, 崔鹤, 王境堂, 赵鑫鹏, 苗钧魁, 刘小芳, 赵宪勇, 冷凯良. 南极磷虾油中总砷含量及砷形态分析[J]. 分析化学, 2016, 44(5): 767-772. doi: 10.11895/j.issn.0253-3820.150942 shu

Citation: WANG Song, LI Ke, CUI He, WANG Jing-Tang, ZHAO Xin-Peng, MIAO Jun-Kui, LIU Xiao-Fang, ZHAO Xian-Yong, LENG Kai-Liang. Quantitative Analysis of Total and Different Species of Arsenic in Oil of Antarctic Krill (Euphausia Superba)[J]. Chinese Journal of Analytical Chemistry, 2016, 44(5): 767-772. doi: 10.11895/j.issn.0253-3820.150942 shu

南极磷虾油中总砷含量及砷形态分析

王松 ^1, ,
LI Ke ^2, ,
崔鹤 ^3, ,
王境堂 ^3, ,
赵鑫鹏 ^1, ,
苗钧魁 ^1, ,
刘小芳 ^1, ,
赵宪勇 ^1, ,
冷凯良 ^1,

1.
中国水产科学研究院黄海水产研究所, 青岛 266071;
2.
Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824 U. S. A;
3.
山东出入境检验检疫局, 青岛 266002
基金项目:
本文系青岛海洋科学与技术国家实验室蓝色生物资源开发利用项目(No.2015ASKJ02)和农业部南极海洋生物资源开发利用项目资助

摘要: 南极磷虾油富含Omega-3磷脂而具有强大的保健功能,但由于其砷含量较高,在保健品领域的应用受到一定限制。由于南极磷虾油中含有大量的磷脂质,采用常规的提取方法分析难以对其砷形态进行准确定量分析。本研究采用高效液相色谱-电感耦合等离子体质谱((HPLC-ICP-MS)联用技术建立了针对砷甜菜碱(AsB)、二甲基砷酸(DMA)、一甲基砷酸(MMA)、三价砷As(Ⅲ)、五价砷As(Ⅲ)5种砷形态的分析方法。采用C₁₈固相萃取进行样品脱脂前处理,采用阴离子交换色谱IonPac AS9-H阴离子分析柱(250 mm×4 mm),流动相采用38 mmol/L Na₂SO₄+15 mmol/L乙酸钠;流速0.6 mL/min,流速1 mL/min,进行5种形态砷化合物的分析。结果表明,5种砷化合物的线性范围为0.5-500μg/L,相关系数R>0.9993.检出限(LOD)为0.1~0.2μg/L,定量限(LOQ)为1.5~2.6μg/L,RSD<5%。加标回收率为88.9%~106.3%。样品的测定结果显示,南极磷虾油中砷形态的主要存在形式为无毒的砷甜菜碱,因此可以安全地应用于食品及保健品。

关键词:

English

Quantitative Analysis of Total and Different Species of Arsenic in Oil of Antarctic Krill (Euphausia Superba)

1.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2.
Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824 U. S. A;
3.
Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China
Received Date: 25 November 2015
Revised Date: 04 January 2016

Abstract: Arsenic is a toxic metalloid that is abundant in many marine species, including Antartic krill. Krill oil is being developed for the health-food and dietary supplement because it possesses of omega-3 fatty acids and phospholipid-derived fatty acids; however, it was limited due to its multiple arsenic species residues. Here, we presented a high performance liquid chromatography-inductively coupled plasma mass spectrometric (HPLC-ICP-MS) method for the simultaneous, sensitive, and rapid quantification of five arsenic metabolites including organic and inorganic forms, then the method was applied to the analysis of arsenic species in krill oil. The analytes were separated on a hydroxide-selective anion-exchange column with sodium carbonate (38 mmol/L) and sodium acetate (15 mmol/L) as mobile phase at a flow rate of 0.6 mL/min for 15 min. Arsenic analytes were monitored with a triple quadrupole ICP mass spectrometer. Validation revealed that the method had a linear range from 0.5 to 500 μg/L with R>0.9993. The LOD was 0.1-0.2 μg/L and LOQ was 1.5-2.6 μg/L with RSD<5%. The standard addition method displayed a recovery range from 88.9% to 106.3%. Application of the method to arsenic species analysis in krill oil indicated that krill oil was a safe food or diet supplement.

Key words:

Antartic krill oil
/ Arsenic
/ Speciation analysis
/ High performance liquid chromatography-inductively coupled plasma mass spectrometry

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沈阳化工大学材料科学与工程学院沈阳 110142

南极磷虾油中总砷含量及砷形态分析

English

Quantitative Analysis of Total and Different Species of Arsenic in Oil of Antarctic Krill (Euphausia Superba)

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

南极磷虾油中总砷含量及砷形态分析

English

Quantitative Analysis of Total and Different Species of Arsenic in Oil of Antarctic Krill (Euphausia Superba)

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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