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DNA片段分选微流控芯片发展现状

李哲煜 ,  孙凯 ,  张笑颜 ,  刘绍琴 ,  江雷 ,  任南琪

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引用本文: 李哲煜, 孙凯, 张笑颜, 刘绍琴, 江雷, 任南琪. DNA片段分选微流控芯片发展现状[J]. 分析化学, 2016, 44(4): 569-578. doi: 10.11895/j.issn.0253-3820.150589 shu
Citation:  LI Zhe-Yu, SUN Kai, ZHANG Xiao-Yan, LIU Shao-Qin, JIANG Lei, REN Nan-Qi. Advance in Microfluidic Devices for Fractionation of DNA Fragments[J]. Chinese Journal of Analytical Chemistry, 2016, 44(4): 569-578. doi: 10.11895/j.issn.0253-3820.150589 shu

DNA片段分选微流控芯片发展现状

  • 1.

    哈尔滨工业大学城市水资源与水环境国家重点实验室, 哈尔滨 150090

    • 通讯作者: 孙凯
  • 基金项目:

    本文系城市水资源与水环境国家重点实验室开放基金(No.2011TS02)资助 (No.2011TS02)

摘要: 新一代测序速度越来越快,费用不断降低,但整个流程中还有不少瓶颈。最耗时且影响测序准确度和重复性的环节之一,就是在文库制备过程中,对打断的大量基因组DNA片段进行基于传统凝胶电泳的手工操作筛选。近几年市场上出现了几种目标片段自动分选仪器,并迅速被多家国际知名测序中心引入测试,从而引起了广泛关注。本文评述了DNA片段分选发展经历,包括凝胶电泳、毛细管电泳、特别是微流控芯片在分选精度和通量上的不断提升,并简要论述了DNA分选目前尚存在的问题。

English

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  • 收稿日期:  2015-07-23
  • 网络出版日期:  2015-09-28
通讯作者: 陈斌, bchen63@163.com
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