液相芯片技术同时检测莱克多巴胺、盐酸克伦特罗和沙丁胺醇

引用本文: 梁世正, 潘家荣, 张弛, 冯涛, 傅丽丽. 液相芯片技术同时检测莱克多巴胺、盐酸克伦特罗和沙丁胺醇[J]. 分析化学, 2016, 44(4): 640-646. doi: 10.11895/j.issn.0253-3820.150172 shu

Citation: LIANG Shi-Zheng, PAN Jia-Rong, ZHANG Chi, FENG Tao, FU Li-Li. Simultaneous Detection of Ractopamine, Clenbuterol and Salbutamol by Suspension Array Technology[J]. Chinese Journal of Analytical Chemistry, 2016, 44(4): 640-646. doi: 10.11895/j.issn.0253-3820.150172 shu

液相芯片技术同时检测莱克多巴胺、盐酸克伦特罗和沙丁胺醇

梁世正 ^1, ,
潘家荣 ^{1,
,} ,
张弛 ^1,2, ,
冯涛 ^1, ,
傅丽丽 ^1,

1.
¹ 中国计量学院浙江省海洋食品品质及危害物控制技术重点实验室, 海洋食品加工质量控制技术与仪器国家地方联合工程实验室, 310018 杭州;
2.
² 重庆市计量质量检测研究院六分院, 重庆 408099

通讯作者: 潘家荣

基金项目:
本文系国家质检公益专项(No.201210086)资助 (No.201210086)

摘要: 建立了一种同时检测兽药莱克多巴胺(RAC)、克伦特罗(CL)、沙丁胺醇(SAL)的多残留的新方法。采用液相悬浮芯片技术,基于间接竞争法的原理,将3种兽药抗原分别偶联到不同的荧光微球上作为探针,以藻红蛋白(PE)荧光标记二抗为信号,通过优化实验条件,分别建立3种兽药的标准曲线。基于特异性识别实验,建立3种兽药同时检测的标准曲线。结果表明,同时检测的3条曲线均呈现良好的线性相关,线性相关系数(R²)均大于0.99,RAC,CL和SAL的检测范围分别为1-500μg/L,0.1-500μg/L,1-100μg/L,检出限分别为0.68,0.095和0.88μg/L。与其它结构类似物的交叉反应率均低于1.5%,实际样品中的加标回收率令人满意。

关键词:

English

Simultaneous Detection of Ractopamine, Clenbuterol and Salbutamol by Suspension Array Technology

1.
¹ China Jiliang University, Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province, National & Local United Engineering Lab of Quality Controlling Technology and Instrumentation for Marine Food, Hangzhou 310018, China;
2.
² Chongqing Academy of Metrology and Quality Inspection of Sixth Branch, Chongqing 408099, China

Corresponding author: 潘家荣

Received Date: 03 May 2015
Fund Project:
本文系国家质检公益专项(No.201210086)资助

Abstract: A multi-residue analytical method was established for the simultaneous detection of ractopamine(RAC), clenbuterol(CL) and salbutamol(SAL) by suspension array technology. Based on the principle of indirect competitive enzyme linked immunosorbent assay, three veterinary antigens coupled with different fluorescent microspheres as probes were prepared and the phycoerythrin(PE)-labeled secondary antibodies were used as reporter signal. Standard curves of three veterinary drugs were separately established under optimum conditions. According to the specific recognition experiment, the standard curves for simultaneous detection of those three veterinary drugs were established. Good linearity was obtained in the concentration range of 1-500μg/L, 0.1-500μg/L, 1-100μg/L, and the detection limits were 0.68, 0.095 and 0.884μg/L for RAC, CL, SAL, respectively, with correlation coefficients(R²)>0.99 for all three drugs. The cross-reactivity of other structural analogues was lower than 1.5%. Good recoveries were found at four spiked levels for both single and mixed standards in real samples.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

液相芯片技术同时检测莱克多巴胺、盐酸克伦特罗和沙丁胺醇

通讯作者: 潘家荣

English

Simultaneous Detection of Ractopamine, Clenbuterol and Salbutamol by Suspension Array Technology

Corresponding author: 潘家荣

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CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

液相芯片技术同时检测莱克多巴胺、盐酸克伦特罗和沙丁胺醇

通讯作者: 潘家荣

English

Simultaneous Detection of Ractopamine, Clenbuterol and Salbutamol by Suspension Array Technology

Corresponding author: 潘家荣

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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