基于四氧化三铁磁性纳米粒子的微囊藻毒素免疫传感器研究

引用本文: 张晶晶, 康天放, 鲁理平, 程水源. 基于四氧化三铁磁性纳米粒子的微囊藻毒素免疫传感器研究[J]. 分析化学, 2016, 44(5): 760-766. doi: 10.11895/j.issn.0253-3820.141067 shu

Citation: ZHANG Jing-Jing, KANG Tian-Fang, LU Li-Ping, CHENG Shui-Yuan. Electrochemical Immunosensor for Microcystins Based on Magnetic Ferriferrous Oxide Nanoparticles Modified Electrode[J]. Chinese Journal of Analytical Chemistry, 2016, 44(5): 760-766. doi: 10.11895/j.issn.0253-3820.141067 shu

基于四氧化三铁磁性纳米粒子的微囊藻毒素免疫传感器研究

北京工业大学区域大气复合污染防治北京市重点实验室, 环境与能源工程学院, 北京 100124
基金项目:
本文系教育部博士点基金(No.20131103110011),中国博士后科学基金(No.71005011201501),北京市自然科学基金重点项目(B类)(No.KZ201110005006)资助

摘要: 采用化学共沉淀法制备四氧化三铁(Fe₃O₄)磁性纳米粒子(MNPs),依次用3-氨基丙基三乙氧基硅烷(APTS)、丁二酸酐(SAH)对Fe₃O₄ MNPs表面进行修饰,得到羧基功能化的核壳型磁性纳米粒子(Fe₃O₄@APTS·SAH MNPs),分别采用透射电镜(TEM)、磁滞回线、X射线光电子能谱(XPS)和傅里叶红外光谱(FTIR)对其进行了表征。将此纳米粒子修饰在自制的磁性玻碳电极(MGCE)表面,用1-(3-二氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC)和N-羟基琥珀酰亚胺(NHS)活化纳米粒子表面的羧基,通过与氨基的共价交联,将抗微囊藻毒素-(亮氨酸-精氨酸)(MCLR)抗体(anti-MCLR)固定于该修饰电极上,用牛血清白蛋白(BSA)封闭非特异性吸附位点,构建了一种检测MCLR的电流型免疫传感器。采用直接竞争免疫反应模式,在标记物辣根过氧化物酶(HRP)的MCLR(MCLR-HRP)存在下,利用差分脉冲伏安法(DPV)测定溶液中的微囊藻毒素。在优化的实验条件下,免疫传感器对MCLR的线性测定范围为0.05~100μg/L,检出限为0.01μg/L(S/N=3)。构建的免疫传感器呈现出良好的重现性、稳定性和特异性。将本传感器用于实际水样的测定,加标回收率为94.3%~99.5%。

关键词:

English

Electrochemical Immunosensor for Microcystins Based on Magnetic Ferriferrous Oxide Nanoparticles Modified Electrode

Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China
Received Date: 08 April 2015
Revised Date: 17 December 2015
Fund Project:
This work was supported by the Doctoral Fund of China Education Ministry (No. 20131103110011), the China Postdoctoral Science Foundation (No.71005011201501), and the key program of Beijing Natural Science Foundation (No. KZ201110005006)

Abstract: An immunosensor for detection of microcystin-(leucine-arginine) (MCLR) was constructed. Chemical co-precipitation method was used to synthesize ferriferrous oxide magnetic nanoparticles (Fe₃O₄ MNPs). The Fe₃O₄ MNPs were modified with 3-aminopropyltriethoxysilane (APTS) and succinic anhydride (SAH) in turn to generate carboxyl-functionalized core-shell MNPs (Fe₃O₄@APTS·SAH MNPs), which were characterized by transmission electron microscope (TEM), hysteresis loop, X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR), respectively. The Fe₃O₄@APTS·SAH MNPs were modified onto the surface of a home-made magnetic glassy carbon electrode (MGCE). Anti-MCLR antibody (anti-MCLR) was immobilized on the modified MGCE by coupling reaction with ethyl-3-(dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). Subsequently, bovine serum albumin (BSA) was used to block the non-specific adsorption sites of the electrode. Direct competitive immunoassay format was adopted. In the presence of horseradish peroxidase-conjugated MCLR (MCLR-HRP), MCLR in solution was determined by differential pulse voltammetry (DPV). Under the optimized conditions, MCLR could be detected within a wide linear range of 0.05-100 μg/L with a detection limit of 0.01 μg/L (S/N=3). Moreover, the immunosnssoe exhibited good reproducibility, stability and selectivity. The immunosensor was applied for the determination of MCLR in real water samples with the recoveries from 94.3% to 99.5%.

Key words:

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基于四氧化三铁磁性纳米粒子的微囊藻毒素免疫传感器研究

English

Electrochemical Immunosensor for Microcystins Based on Magnetic Ferriferrous Oxide Nanoparticles Modified Electrode

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中国化学会秘书处

基于四氧化三铁磁性纳米粒子的微囊藻毒素免疫传感器研究

English

Electrochemical Immunosensor for Microcystins Based on Magnetic Ferriferrous Oxide Nanoparticles Modified Electrode

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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