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Investigations on the degradation of aspartame using high-performance liquid chromatography/tandem mass spectrometry

Jie-Ping Sun ,  Qiang Han ,  Xiao-Qiong Zhang ,  Ming-Yu Ding

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Citation:  Jie-Ping Sun, Qiang Han, Xiao-Qiong Zhang, Ming-Yu Ding. Investigations on the degradation of aspartame using high-performance liquid chromatography/tandem mass spectrometry[J]. Chinese Chemical Letters, 2014, 25(9): 1259-1264. doi: 10.1016/j.cclet.2014.04.012 shu

Investigations on the degradation of aspartame using high-performance liquid chromatography/tandem mass spectrometry

    • 通讯作者: Ming-Yu Ding,
  • 基金项目:

    This project was supported by the National Nature Science Foundation of China (No. 21075074). (No. 21075074)

摘要: Aspartame is a widely used sweetener, the long-term safety of which has been controversial ever since it was accepted for human consumption. It is unstable and can produce some harmful degradation products under certain storage conditions. A high-performance liquid chromatography/tandem mass spectrometry method was developed for the simultaneous analysis of aspartame and its four degradation products, including aspartic acid, phenylalanine, aspartyl-phenylalanine and 5-benzyl-3,6-dioxo-2-piperazieacetic acid in water and in diet soft drinks. Aspartame and its four degradation products were quantified by a matrix matched external standard calibration curve with excellent correlation coefficients. The limits of detection were 0.16-5.8 μg/L, which exhibited higher sensitivity than common methods. This method was rapid, sensitive, specific and capable of eliminating matrix interferences. It was also applied to the study of the degradation of aspartame at various pH and temperatures. The results indicated that aspartame was partly degraded under strong acidic or basic conditions and the extent of degradation increased with increasing temperature.

English

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  • 收稿日期:  2014-02-07
  • 网络出版日期:  2014-04-09
通讯作者: 陈斌, bchen63@163.com
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