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Capillary electrophoresis with laser-induced fluorescence detection of main polyamines and precursor amino acids in saliva

Zhuo Li ,  Yang Zhang ,  Fang-Hong Tong ,  Ting-Ting Jiang ,  Hui-Ping Zheng ,  Jian-Nong Ye ,  Qing-Cui Chu

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Citation:  Zhuo Li, Yang Zhang, Fang-Hong Tong, Ting-Ting Jiang, Hui-Ping Zheng, Jian-Nong Ye, Qing-Cui Chu. Capillary electrophoresis with laser-induced fluorescence detection of main polyamines and precursor amino acids in saliva[J]. Chinese Chemical Letters, 2014, 25(4): 640-644. doi: 10.1016/j.cclet.2014.01.037 shu

Capillary electrophoresis with laser-induced fluorescence detection of main polyamines and precursor amino acids in saliva

    • 通讯作者: Qing-Cui Chu,
  • 基金项目:

    This work was supported by the Natural Science Foundation of China (No.21205042) (No.21205042)

    Equipment (No. 2011YQ15007205) (No. 2011YQ15007205)

    the Daxia Foundation of ECNU (No. 2012DX-185). (No. 2012DX-185)

摘要: A hollow-fiber liquid-phasemicroextraction (HF-LPME) method has been developed for the purification and preconcentration of biogenic polyamines and their precursor amino acids in human saliva. Putrescine (Put), cadaverine (Cad), spermidine (Spe), ornithine (Orn), lysine (Lys), and arginine (Arg) were determined by the CE-LIF detection after microextraction. Several factors that affect extraction efficiency, separation, and detection were investigated. Under the optimum conditions, six analytes could achieve baseline separation within 30 min, exhibiting a linear calibration at three orders of magnitude (r2 > 0.998); the obtained enrichment factors of HF-LPME were between 19 (for Orn) and 218 (for Cad), and the LODs were in the range of 0.0072-0.26 nmol/L. The proposed HF-LPME/CE-LIF method has been successfully applied for the sensitive analyses of the real-world saliva samples collected from healthy volunteers and different patients with oral diseases, providing a potential method for primary non-invasive diagnosis of some oral diseases.

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  • 发布日期:  2014-01-31
  • 收稿日期:  2013-12-12
  • 网络出版日期:  2014-01-07
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