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Low temperature purification method for the determination of abamectin and ivermectin in edible oils by liquid chromatography-tandem mass spectrometry

Jian-Xiang Huang ,  Da-Hai Lu ,  Kai Wan ,  Fu-Hua Wang

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Citation:  Jian-Xiang Huang, Da-Hai Lu, Kai Wan, Fu-Hua Wang. Low temperature purification method for the determination of abamectin and ivermectin in edible oils by liquid chromatography-tandem mass spectrometry[J]. Chinese Chemical Letters, 2014, 25(4): 635-639. doi: 10.1016/j.cclet.2014.01.036 shu

Low temperature purification method for the determination of abamectin and ivermectin in edible oils by liquid chromatography-tandem mass spectrometry

    • 通讯作者: Fu-Hua Wang,
  • 基金项目:

    This research was financially supported by National Natural Science Foundation of China (No. 21305019) (No. 21305019)

    Special Fund for Agro-Scientific Research in the Public Interest (No. 201303088)  (No. 201303088)

    President Fund of Guangdong Academy of Agricultural Sciences (No. 201218). (No. 201218)

摘要: In this study, a method based on low temperature purification (LTP) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of abamectin (ABA) and ivermectin (IVR) in edible oils. ABA and IVR were extracted using conventional liquid-liquid extraction followed by purification via precipitation of interfering fatty components at low temperature without an additional cleanup step. LTP is simple, easy to use, labour-saving and cost effective, and requires reduced amounts of organic solvent. The linear ranges of ABA and IVR were 5- 1000 μg/L using matrix-matched standards. Limits of detection (LOD) and limits of quantification (LOQ) were in the range of 0.1-0.4 μg/kg and 0.3-1.3 μg/kg, respectively. The LOQs were below the strictest maximum residue limits established by Codex Alimentarius Commission. Recoveries at three spiked levels of 10, 20 and 100 μg/kg in peanut oil, corn oil, olive oil, soybean oil and lard ranged from 71.1% to 119.3% with relative standard deviations of 3.2%-10.3%, which were in agreement with those obtained by the solid phase extraction method. The proposed method was utilized in the analysis of 10 edible oil samples from localmarket and neither ABA nor IVR was detected. As far as we know, this is the first time that LTP is applied to the determination of avermectins in edible oils.

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  • 发布日期:  2014-01-31
  • 收稿日期:  2013-11-11
  • 网络出版日期:  2014-01-13
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