引用本文:
梁爱惠, 邹节明, 蒋治良, 王力生, 覃爱苗. 磷钼杂多酸与蛋白质相互作用的共振散射光谱研究[J]. 应用化学,
2002, 19(8): 768-771.
Citation: LIANG Ai-Hui, ZOU Jie-Ming, JIANG Zhi-Liang, WANG Li-Sheng, QIN Ai-Miao. Resonance Scattering Study on the Interaction Between Protein and Phosphomolybdic Acid and Its Analytical Application[J]. Chinese Journal of Applied Chemistry, 2002, 19(8): 768-771.
Citation: LIANG Ai-Hui, ZOU Jie-Ming, JIANG Zhi-Liang, WANG Li-Sheng, QIN Ai-Miao. Resonance Scattering Study on the Interaction Between Protein and Phosphomolybdic Acid and Its Analytical Application[J]. Chinese Journal of Applied Chemistry, 2002, 19(8): 768-771.
磷钼杂多酸与蛋白质相互作用的共振散射光谱研究
English
Resonance Scattering Study on the Interaction Between Protein and Phosphomolybdic Acid and Its Analytical Application
Abstract:
A maximum resonance scattering(RS) peak at 470 nm for the system of protein-phosphomolybdic acid(PMA) was observed. The enhanced resonance scattering signal appeared to be the formation of protein-PMA composite nanoparticles with diameter about 80 nm. A resonance scattering spectroscopic method has been developed for the quantitative determination of human serum albumin (HSA) and casein. In the condition selected, the value of I470 nm was found linear to the concentration of protein in the range of 6.0×10-8~5.0×10-5g/mL, with detection limit about 2.0×10-8~1.0×10-7g/mL. The method has advantages of easy operation, high sensitivity, high accuracy and wide determinination range. Proteins in synthetic samples and human serum samples have been determined by this RS method with satisfactory results.
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Key words:
- phosphomolybdic acid
- / proteins
- / resonance Scattering spectroscopy
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