引用本文:
廉革伟, 刘立岩, 陈默, 赵大庆, 倪嘉缵, 姚笛. 甲状腺素脱碘抗体酶制备半抗原和全抗原的合成与单克隆抗体的制备[J]. 应用化学,
2002, 19(4): 387-389.
Citation: LIAN Ge-Wei, LIU Li-Yan, CHEN Mo, ZHAO Da-Qing, NI Jia-Zuan, YAO Di. Preparation of Deiodinating Abzyme of Thyroxine——Synthesis of Haptens and Antigens and Preparation of Monoclonal Antibody[J]. Chinese Journal of Applied Chemistry, 2002, 19(4): 387-389.
Citation: LIAN Ge-Wei, LIU Li-Yan, CHEN Mo, ZHAO Da-Qing, NI Jia-Zuan, YAO Di. Preparation of Deiodinating Abzyme of Thyroxine——Synthesis of Haptens and Antigens and Preparation of Monoclonal Antibody[J]. Chinese Journal of Applied Chemistry, 2002, 19(4): 387-389.
甲状腺素脱碘抗体酶制备半抗原和全抗原的合成与单克隆抗体的制备
English
Preparation of Deiodinating Abzyme of Thyroxine——Synthesis of Haptens and Antigens and Preparation of Monoclonal Antibody
Abstract:
Three thyroxine haptens were synthesized by protecting amino group of thyroxine with Cu 2+ ion and characterized by 1H NMR, MS, elemental analysis and IR. Thyroxine and the derivatives were coupled with carrier proteins(BSA and Ovalbumin). Thus antigens were prepared and hapten quantities of the antigens were measured by UV spectroscopy at 320 nm. An anti-O-methyl-T4 monoclonal antibody was prepared by immunization, cell fusion and screening.
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Key words:
- cupric ion
- / thyroxine
- / hapten
- / antigen
- / monoclonal antibody
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