引用本文:
周蓉, 范毅, 冯钰錡, 达世禄. 苯胺甲基键合固定相的合成和色谱性能[J]. 应用化学,
2002, 19(1): 66-70.
Citation: ZHOU Rong, FAN Yi, FENG Yu-Qi, DA Shi-Lu. Preparation and Evaluation of N-Methylaniline Bonded Silica for High Performance Liquid Chromatography[J]. Chinese Journal of Applied Chemistry, 2002, 19(1): 66-70.
Citation: ZHOU Rong, FAN Yi, FENG Yu-Qi, DA Shi-Lu. Preparation and Evaluation of N-Methylaniline Bonded Silica for High Performance Liquid Chromatography[J]. Chinese Journal of Applied Chemistry, 2002, 19(1): 66-70.
苯胺甲基键合固定相的合成和色谱性能
摘要:
研究了苯胺甲基键合硅胶固定相的合成及其色谱性能.采用元素分析、红外光谱对固定相的组成进行了表征.以甲醇/水为流动相考察了该固定相的色谱性能.该固定相具有较好的疏水作用和电荷转移能力.多环芳烃在该固定相的保留与正十八烷固定相(ODS)柱相似;硝基苯类、硝基苯酚类和氯苯类的溶质在该固定相保留则存在着电荷转移、偶极-偶极作用.同时对封尾前后该固定相的色谱行为进行了比较.
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关键词:
- 多环芳烃
- / 色谱法
- / 苯胺甲基键合硅胶固定相
English
Preparation and Evaluation of N-Methylaniline Bonded Silica for High Performance Liquid Chromatography
Abstract:
A new bonded stationary phase, N-methylaniline bonded silica for reversed-phase HPLC was synthesized by reacting N-methylanilinetriethoxylsiliane with silica directly. The packing material was characterized by diffuse reflectance infrared Fourier transform spectrometry and elemental analysis. The chromatographic behavior of hydrophobicity, charge-transfer ability, dipole-dipole and hydrogen bonding interaction have been studied on the bonded stationary phase using methanol-water as binary mobile phase. Compared with octadecyl stationary phase(ODS), the new bonded phase was superior in selectivity and separation rate for PAH and it's derivatives, and exhibited the best separation selectivity for acidic aromatic derivatives.
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