引用本文:
邱建丁, 梁汝萍, 邹小勇, 蔡沛祥, 张宝生. 自组装抗-HBs膜免疫电极的研制和应用[J]. 应用化学,
2001, 18(9): 705-708.
Citation: QIU Jian-Ding, LIANG Ru-Ping, ZOU Xiao-Yong, CAI Pei-Xiang, ZHANG Bao-Sheng. The Self-assembled HBsAb Monolayer Gold Electrode and Its Application to HBsAg Determination[J]. Chinese Journal of Applied Chemistry, 2001, 18(9): 705-708.
Citation: QIU Jian-Ding, LIANG Ru-Ping, ZOU Xiao-Yong, CAI Pei-Xiang, ZHANG Bao-Sheng. The Self-assembled HBsAb Monolayer Gold Electrode and Its Application to HBsAg Determination[J]. Chinese Journal of Applied Chemistry, 2001, 18(9): 705-708.
自组装抗-HBs膜免疫电极的研制和应用
English
The Self-assembled HBsAb Monolayer Gold Electrode and Its Application to HBsAg Determination
Abstract:
A gold electrode modified by hepatitis B surface antibody covalently bound to selfassembled thiourea monolayer has been fabricated to detect the hepatitis B surface antigen.The selfassembled monolayer was formed by spontaneous adsorption of thiourea on gold surface and used as a substrate to be modified by anti-hepatitis B surface antigen through glutaraldehyde amide reactions.The modification procedure was electrochemically monitored by characterization of the electrode surface.The immunoelectrode and saturated calomel electrode were dipped together into the serum samples diluted by PBS buffer solution (pH=7.4) for the potentiometric determination.More than 87.5% of the results of the human serum samples obtained by this method were in agreement with those obtained by enzyme linked immunosorbent assay(ELISA).Calibration graphs were linear for HBsAg in range of 6~320 μg/L with correlation coefficient of 0 998 and detection limit of 4 μg/L.The immunoelectrode prepared has good stability and high sensitivity.
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Key words:
- hepatitis B surface antigen
- / immunoelectrode
- / self-assembled monolayer
- / thiourea
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