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Citation: Peng Tuzhi, Zhu Fangmeng, Yang Liju, He Qiaohong, Cheng Qiong. Potentiometric Determination of α-Fetoprotein (AFP) Using a Non-labeled Antibody Immunosensor[J]. Chinese Journal of Applied Chemistry, ;1998, 15(1): 47-49. shu

Potentiometric Determination of α-Fetoprotein (AFP) Using a Non-labeled Antibody Immunosensor

Department of Chemistry, Hangzhou University, Hangzhou 310028

Received Date: 9 April 1997
Revised Date: 10 September 1997

The anti-AFP antibody is immobilized on the surface of PVC-BSA(bovine serum albumin) membrane, which has been activated by NH₂OH · HCl solution. The immunosen-sor consists of such a membrane with added AFP and Ag-AgCl internal electrode. It showed linear response over concentration range of 10^-300μg/L and potential difference(△E) of 15~20 mV for 300μg/L of AFP. Other common antigens in human serum do not interfere with AFP's determination.
- α-fetoprotein,
- potentiometry,
- immunosensor
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