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Citation: SUN Shuang-Jiao, LIANG Ai-Hui, JIANG Zhi-Liang, ZHONG Fu-Xin. A Simple and Sensitive Immune Resonance Scattering Spectral Assay for Determination of Fibrinogen[J]. Chinese Journal of Applied Chemistry, ;2009, 26(4): 451-455. shu

A Simple and Sensitive Immune Resonance Scattering Spectral Assay for Determination of Fibrinogen

1.
a School of Environment and Resource, Guangxi Normal University, Guilin 541004;
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b Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004

Corresponding author: JIANG Zhi-Liang,
Received Date: 29 May 2008
Available Online: 11 September 2008
Fund Project: 国家自然科学基金(20365001,20667001) (20365001,20667001)广西新世纪十百千人才计划(2004206)资助项目 (2004206)

In a pH 6.2 Na₂HPO₄-NaH₂PO₄ buffer solution,the immune reaction of goat anti-human fibrinogen serum and human fibrinogen(Fg) forms immune complex particles that exhibit a stronger resonance scattering peak at 340 nm.The resonance scattering intensity at 340 nm was enhanced with the increase of concentration of Fg.There is a linear relation in resonance scattering intensity ΔI_{340 nm} to the Fg concentration c in a range of 1.33×10^-7~5.33×10^-6 g/mL.The regressed linear equation is ΔI_{340 nm}=12.17c+3.35,the relation coefficient is 0.998 6.The detection limit(3σ) is achieved at 2.8×10^-8 g/mL Fg.Influence of 18 substances on the determination was examined.Results indicate that this assay has a good selectivity.The proposed method was applied to the determination of Fg in plasma,with a relative standard deviation of 2.2%~2.3%.The result is in agreement with the Fg content in normal plasma.
- fibrinogen,
- immunoreaction,
- resonance scattering spectral assay
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