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Citation: HUANG Bao-Mei, YAO Cheng-Wei, BIAN Qing-Quan, WANG Zhi-Guo, MO Jin-Yuan. Determination of Barbalion in Aloe by Capillary Electrophoresis Coupled with Amperometric Detection[J]. Chinese Journal of Applied Chemistry, ;2009, 26(3): 302-305. shu

Determination of Barbalion in Aloe by Capillary Electrophoresis Coupled with Amperometric Detection

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a Department of Chemistry, Mianyang Normal College, Mianyang 621000;
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b Institute of Facility Design and Instrumentation, CARDC, Mianyang;
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c School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou

Corresponding author: HUANG Bao-Mei,
Received Date: 9 March 2008
Available Online: 14 June 2008
Fund Project: 四川省教育厅资助项目(07zb041) (07zb041)

Barbalion in Aloe was determined by capillary electrophoresis coupled with amperometric detection(CE-AD).The pH,type and concentration of the running buffer,the detection potential,the applied voltage and the sampling time were selected as the investigated variables.Under the optimized conditions: a running buffer composed of 30 mmol/L Na₂B₄O₇ buffer at pH 9.5,separation voltage at 15 kV,injection time 10 seconds,2.5% methanol additive and detection potential-0.8 V,a linearity existed between peak area(Y) and mass concentration of Barbalion(c) in a range of 0.05～50 mg/L,r=0.999 4,and the determination limit was 0.01 mg/L.
- capillary electrophoresis,
- Barbalion,
- amperometric detection
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