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Citation: Deng Huimin, Li Jun, Lai Zhihui, Zhao Shankai. Determination of Nucleotide Molecules by Matrixassisted Laser Desorption/Ionization Mass Spectrometry[J]. Chinese Journal of Applied Chemistry, ;1999, 16(1): 95-97. shu

Determination of Nucleotide Molecules by Matrixassisted Laser Desorption/Ionization Mass Spectrometry

1.
Instrumental Analysis and Research Centre, Zhongshan University, Guangzhou 510275

Received Date: 4 May 1998
Available Online: 17 September 1998
Fund Project:

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry has been used to determine the molecular weights of DNA oligomers d(T)₆ and d(C)₁₀.The results showed that 3-hydroxypicolinic acid was effective matrix in comparision with the mixture of 3-hydroxy picolinic acid and picolinic acid, 2, 5-dihydroxybenzoic acid, α-cyano-4-hydroxycinnamic acid, 2, 5-dihydroxyacetophenone and 2, 4, 6-trihydroxyacetophenone.NH₄⁺ ion exchane resin and diammonium hydrogen citrate were used successfully to eliminate the influences of Na⁺, K⁺, Ca²⁺, Mg²⁺ ions.
- matrix-assisted laser deso rption/ionization mass spectrometry,
- nucleotide,
- molecular weight determination
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