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Citation: LIAN Ge-Wei, LIU Li-Yan, CHEN Mo, ZHAO Da-Qing, NI Jia-Zuan, YAO Di. Preparation of Deiodinating Abzyme of Thyroxine——Synthesis of Haptens and Antigens and Preparation of Monoclonal Antibody[J]. Chinese Journal of Applied Chemistry, ;2002, 19(4): 387-389. shu

Preparation of Deiodinating Abzyme of Thyroxine——Synthesis of Haptens and Antigens and Preparation of Monoclonal Antibody

LIAN Ge-Wei ^a,, ,
LIU Li-Yan ^a ,
CHEN Mo ^a ,
ZHAO Da-Qing ^a ,
NI Jia-Zuan ^a ,
YAO Di ^b

1.
a Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022;
2.
b Department of Thyroid Function, No.1 Accessary Hospital of University of Bethune Medical Sciences, Changchun

Corresponding author: LIAN Ge-Wei,
Received Date: 15 August 2001
Available Online: 4 December 2001
Fund Project: 中国科学院重大基金资助项目(29701005) (29701005)

Three thyroxine haptens were synthesized by protecting amino group of thyroxine with Cu ²⁺ ion and characterized by ¹H NMR, MS, elemental analysis and IR. Thyroxine and the derivatives were coupled with carrier proteins(BSA and Ovalbumin). Thus antigens were prepared and hapten quantities of the antigens were measured by UV spectroscopy at 320 nm. An anti-O-methyl-T₄ monoclonal antibody was prepared by immunization, cell fusion and screening.
- cupric ion,
- thyroxine,
- hapten,
- antigen,
- monoclonal antibody
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